Burkholderia mallei is the causative agent of glanders, a zoonosis listed by the World Group for Animal Well being as of obligatory notification. On this work, a comparability of three qPCR protocols was made, two of them based mostly on articles by different authors and one standardized in home, this final one aiming at a genomic area that doesn’t exist in different species of the Burkholderia genus. All qPCRs confirmed excessive effectivity and good repeatability. Nevertheless, reactions with Cq between 36 and 40 had been thought-about suspicious and unreliable, requiring better scientific standards to investigate the outcomes.
Knowledge on the optimization of an archaea-specific probe-based qPCR assay
Estimation of archaeal numbers by use of fluorescent DNA binding dyes is difficult, as a result of primers concentrating on the archaeal 16SrRNA genes readily additionally bind to bacterial 16S rRNA gene sequences, particularly when the relative abundance of micro organism is bigger than that of archaea. With the intention to enhance specificity, we optimized a fluorescent probe-based assay utilizing beforehand printed archaeal primers and probe. The assay was examined on genomic DNA of pure bacterial and archaeal cultures and optimized utilizing PCR amplicons of the archaeal pure cultures.
The used bacterial strains confirmed slight amplification utilizing the fluorescent dye assay, whereas all archaeal strains may very well be amplified with the archaea primers used. On account of variations in genome dimension and variety of 16S rRNA gene copies between the examined archaeal strains, the amplification degree different significantly between the strains.
Due to this fact, we additionally examined the amplification utilizing PCR amplified fragments of the archaeal 16S rRNA genes. The checks with the archaeal 16S rRNA gene amplicons confirmed good amplification, though the amplification effectivity nonetheless different between archaeal strains. The qPCR assay was used to estimate the archaeal numbers in course of water of a multi-metal mine’s metallurgical plant [1] and might be utilized in comparable future microbiological evaluation included within the H2020 ITERAMS venture (Grant settlement# 730480).
Transcriptome analyses of urine RNA reveal tumor markers for human bladder most cancers: validated amplicons for RT-qPCR-based detection
Non-invasive scientific diagnostics of bladder most cancers is possible by way of a set of chemically distinct molecules together with macromolecular tumor markers comparable to polypeptides and nucleic acids. When it comes to tumor-related aberrant gene expression, RNA transcripts are the first indicator of tumor-specific gene expression as for polypeptides and their metabolic merchandise happen subsequently.
Thus, in case of bladder most cancers, urine RNA represents an early doubtlessly helpful diagnostic marker. Right here we describe a scientific deep transcriptome evaluation of consultant swimming pools of urine RNA collected from wholesome donors versus bladder most cancers sufferers in response to established SOPs. This evaluation revealed RNA marker candidates reflecting coding sequences, non-coding sequences, and round RNAs. Subsequent, we designed and validated PCR amplicons for a set of novel marker candidates and examined them in human bladder most cancers cell strains.
We recognized linear and round transcripts of the S100 Calcium Binding Protein 6 (S100A6) and translocation related membrane protein 1 (TRAM1) as extremely promising potential tumor markers. This work strongly suggests exploiting urine RNAs as diagnostic markers of bladder most cancers and it suggests particular novel markers. Additional, this research describes an entry into the tumor-biology of bladder most cancers and the event of gene-targeted therapeutic medication.
Analysis of RT-qPCR and Loop-Mediated Isothermal Amplification (LAMP) Assays for the Detection of SARS-CoV-2 in Argentina
Our purpose was to judge the analytical and scientific efficiency of the SARS-CoV-2 molecular detection kits utilized in Argentina. 9 real-time reverse-transcription polymerase chain response (RT-qPCR) and three reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assays had been evaluated utilizing the World Well being Group (WHO) beneficial check as reference methodology. A secondary commonplace calibrated for the E, N and RdRp genes towards the Pan American Well being Group-World Well being Group-Worldwide Normal was used to calculate the restrict of detection (LoD).
Probe qPCR SuperMix |
20-abx098040 |
Abbexa |
-
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Green qPCR SuperMix |
abx098031-100l |
Abbexa |
100 µl |
EUR 675 |
Green qPCR SuperMix |
abx098031-200l |
Abbexa |
200 µl |
EUR 912.5 |
Pro QPCR SuperMix Kit - ROX premixed |
K5056200 |
Biochain |
200 reactions |
EUR 180 |
Pro QPCR SuperMix Kit - ROX premixed |
K5056400 |
Biochain |
400 reactions |
EUR 326 |
Green qPCR SuperMix UDG |
20-abx098032 |
Abbexa |
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Top Green qPCR SuperMix |
20-abx098033 |
Abbexa |
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Tip Green qPCR SuperMix |
20-abx098034 |
Abbexa |
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Green qPCR SuperMix UDG |
abx098032-100l |
Abbexa |
100 µl |
EUR 687.5 |
Green qPCR SuperMix UDG |
abx098032-200l |
Abbexa |
200 µl |
EUR 912.5 |
Top Green qPCR SuperMix |
abx098033-100l |
Abbexa |
100 µl |
EUR 262.5 |
Top Green qPCR SuperMix |
abx098033-200l |
Abbexa |
200 µl |
EUR 487.5 |
Tip Green qPCR SuperMix |
abx098034-100l |
Abbexa |
100 µl |
EUR 262.5 |
Tip Green qPCR SuperMix |
abx098034-200l |
Abbexa |
200 µl |
EUR 437.5 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058200 |
Biochain |
200 reactions |
EUR 211 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
K5058400 |
Biochain |
400 reactions |
EUR 354 |
Library Quantification qPCR SuperMix |
20-abx098896 |
Abbexa |
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Library Quantification qPCR SuperMix |
abx098896-100l |
Abbexa |
100 µl |
EUR 700 |
Library Quantification qPCR SuperMix |
abx098896-200l |
Abbexa |
200 µl |
EUR 962.5 |
EnTurbo™ probe qPCR SuperMix |
EQ017-25mL |
ELK Biotech |
25mL |
EUR 240 |
EnTurbo™ probe qPCR SuperMix |
EQ017-5mL |
ELK Biotech |
5mL |
EUR 78 |
EnTurbo™ probe qPCR SuperMix |
EQ017 |
ELK Biotech |
5mL |
EUR 240 |
Top Green qPCR SuperMix (+Dye II) |
20-abx098890 |
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Tip Green qPCR SuperMix (+Dye II) |
20-abx098891 |
Abbexa |
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Top Green qPCR SuperMix (+Dye II) |
abx098890-100l |
Abbexa |
100 µl |
EUR 262.5 |
Top Green qPCR SuperMix (+Dye II) |
abx098890-200l |
Abbexa |
200 µl |
EUR 487.5 |
Tip Green qPCR SuperMix (+Dye II) |
abx098891-100l |
Abbexa |
100 µl |
EUR 262.5 |
Tip Green qPCR SuperMix (+Dye II) |
abx098891-200l |
Abbexa |
200 µl |
EUR 437.5 |
EnTurbo™ SYBR Color qPCR SuperMix |
EQ036-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix |
EQ036-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Color qPCR SuperMix |
EQ036 |
ELK Biotech |
5mL |
EUR 340 |
cDNA Synthesis SuperMix for qPCR |
20-abx09801920ulSystems |
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HyperScript RT SuperMix for qPCR |
K1074-100 |
ApexBio |
100 rxn (20 uL/rxn) |
EUR 280 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR |
K1074-50 |
ApexBio |
50 rxn (20 uL/rxn) |
EUR 150 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
cDNA Synthesis SuperMix for qPCR |
abx098019-100l |
Abbexa |
100 µl |
EUR 825 |
cDNA Synthesis SuperMix for qPCR |
abx098019-1ml |
Abbexa |
1 ml |
EUR 2387.5 |
cDNA Synthesis SuperMix for qPCR |
abx098019-200l |
Abbexa |
200 µl |
EUR 1000 |
HiScript II Q RT SuperMix for qPCR |
R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 145 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
EQ035-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
EQ035-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
EQ035 |
ELK Biotech |
5mL |
EUR 340 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 861.6 |
|
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-100l |
Abbexa |
100 µl |
EUR 1025 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-1ml |
Abbexa |
1 ml |
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cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-200l |
Abbexa |
200 µl |
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HiScript II Q Select RT SuperMix for qPCR |
R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 145 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
R423-01 |
Vazyme |
100rxns(20µl/rxn) |
EUR 326 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
R423-00-10rxns |
Vazyme |
10 rxns |
EUR 1.09 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
R423-01-100rxns |
Vazyme |
100 rxns |
EUR 337.36 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 217.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
abx098860-100l |
Abbexa |
100 µl |
EUR 1012.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
abx098860-1ml |
Abbexa |
1 ml |
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Uni Cell to cDNA Synthesis SuperMix for qPCR |
abx098860-200l |
Abbexa |
200 µl |
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HiScript III RT SuperMix for qPCR (+gDNA wiper) |
R323-00-10rxns |
Vazyme |
10 rxns |
EUR 1.09 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
R323-01-100rxns |
Vazyme |
100 rxns |
EUR 225.22 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
EQ033-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
EQ033-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
EQ033 |
ELK Biotech |
5mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
EQ034-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
EQ034-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
EQ034 |
ELK Biotech |
5mL |
EUR 340 |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
K1174-100 |
ApexBio |
100 rxns |
EUR 304 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
K1174-50 |
ApexBio |
50 rxns |
EUR 176 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HiScript II Q RT SuperMix for qPCR (+gDNA wiper) |
R223-01 |
Vazyme |
100rxns(20µl/rxn) |
EUR 159.5 |
HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) |
R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 159.5 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054200 |
Biochain |
200 reactions |
EUR 468 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054400 |
Biochain |
400 reactions |
EUR 711 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
R333-01 |
Vazyme |
100 rxns (21 μl/rxn) |
EUR 217.5 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
R333-00-10rxns |
Vazyme |
10 rxns |
EUR 1.09 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
R333-01-100rxns |
Vazyme |
100 rxns |
EUR 225.22 |
Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054002 |
Biochain |
200 rxn |
EUR 468 |
Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054004 |
Biochain |
400 rxn |
EUR 711 |
PCR SuperMix |
20-abx098887 |
Abbexa |
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2X PCR SuperMix |
MB200-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 11 |
2X PCR SuperMix |
MBS402025-100Reactions |
MyBiosource |
100Reactions |
EUR 175 |
2X PCR SuperMix |
MBS402025-4Reactions |
MyBiosource |
4Reactions |
EUR 160 |
PCR SuperMix (-dye) |
20-abx098002 |
Abbexa |
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PCR SuperMix (+dye) |
20-abx098003 |
Abbexa |
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PCR SuperMix (-dye) |
abx098002-100l |
Abbexa |
100 µl |
EUR 237.5 |
PCR SuperMix (-dye) |
abx098002-200l |
Abbexa |
200 µl |
EUR 337.5 |
PCR SuperMix (+dye) |
abx098003-100l |
Abbexa |
100 µl |
EUR 237.5 |
PCR SuperMix (+dye) |
abx098003-200l |
Abbexa |
200 µl |
EUR 337.5 |
Plus PCR SuperMix |
20-abx098888 |
Abbexa |
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Evo? cDNA Supermix |
M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
each |
EUR 320.4 |
Plus PCR SuperMix |
abx098888-100l |
Abbexa |
100 µl |
EUR 300 |
Plus PCR SuperMix |
abx098888-200l |
Abbexa |
200 µl |
EUR 612.5 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
each |
EUR 346.8 |
Probe Direct SuperMix |
abx461057-1096tests |
Abbexa |
10 × 96 tests |
Ask for price |
Probe Direct SuperMix |
abx461057-596tests |
Abbexa |
5 × 96 tests |
EUR 2800 |
Probe Direct SuperMix |
abx461057-96tests |
Abbexa |
96 tests |
EUR 812.5 |
cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
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cDNA Synthesis SuperMix |
abx098014-100l |
Abbexa |
100 µl |
EUR 725 |
cDNA Synthesis SuperMix |
abx098014-1ml |
Abbexa |
1 ml |
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cDNA Synthesis SuperMix |
abx098014-200l |
Abbexa |
200 µl |
EUR 812.5 |
T PCR SuperMix (-dye) |
20-abx098005 |
Abbexa |
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T PCR SuperMix (+dye) |
20-abx098006 |
Abbexa |
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T PCR SuperMix (-dye) |
abx098005-100l |
Abbexa |
100 µl |
EUR 262.5 |
T PCR SuperMix (-dye) |
abx098005-200l |
Abbexa |
200 µl |
EUR 462.5 |
T PCR SuperMix (+dye) |
abx098006-100l |
Abbexa |
100 µl |
EUR 262.5 |
T PCR SuperMix (+dye) |
abx098006-200l |
Abbexa |
200 µl |
EUR 462.5 |
AmpHS 2X PCR SuperMix |
MB203-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 11 |
EasyPfu PCR SuperMix (-dye) |
20-abx098009 |
Abbexa |
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FastPfu PCR SuperMix (-dye) |
20-abx098010 |
Abbexa |
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FastPfu PCR SuperMix (-dye) |
abx098010-100l |
Abbexa |
100 µl |
EUR 300 |
FastPfu PCR SuperMix (-dye) |
abx098010-200l |
Abbexa |
200 µl |
EUR 575 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055200 |
Biochain |
200 reactions |
EUR 427 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055400 |
Biochain |
400 reactions |
EUR 682 |
Library Amplification SuperMix |
20-abx098894 |
Abbexa |
-
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Library Amplification SuperMix |
abx098894-100l |
Abbexa |
100 µl |
EUR 737.5 |
Library Amplification SuperMix |
abx098894-200l |
Abbexa |
200 µl |
EUR 1137.5 |
OneScriptcDNA Synthesis SuperMix |
G451 |
ABM |
25 x 20 ul reactions |
EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
G452 |
ABM |
100 x 20 ul reactions |
EUR 169.2 |
PCR SuperMix for PAGE (+dye) |
20-abx098004 |
Abbexa |
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PCR SuperMix for PAGE (+dye) |
abx098004-100l |
Abbexa |
100 µl |
EUR 237.5 |
PCR SuperMix for PAGE (+dye) |
abx098004-200l |
Abbexa |
200 µl |
EUR 337.5 |
Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055002 |
Biochain |
200 rxn |
EUR 426 |
Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055004 |
Biochain |
400 rxn |
EUR 682 |
OneScriptPlus cDNA Synthesis SuperMix |
G453 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
G454 |
ABM |
100 x 20 ul reactions |
EUR 202.8 |
OmniPCR Supermix w Fluorescent dye |
MBA01-0100 |
Bio-Helix |
100 rxns (2.5ml) |
EUR 16 |
EnTurbo™ SYBR Green PCR SuperMix |
EQ001-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix |
EQ001-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Green PCR SuperMix |
EQ001 |
ELK Biotech |
5mL |
EUR 340 |
Green Two-Step qRT-PCR SuperMix |
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 777.6 |
|
Green One-Step qRT-PCR SuperMix |
20-abx09803820ulSystems |
Abbexa |
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Probe One-Step qRT-PCR SuperMix |
20-abx09804220ulSystems |
Abbexa |
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Green Two-Step qRT-PCR SuperMix |
abx098035-100l |
Abbexa |
100 µl |
EUR 925 |
Green Two-Step qRT-PCR SuperMix |
abx098035-1ml |
Abbexa |
1 ml |
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Green Two-Step qRT-PCR SuperMix |
abx098035-200l |
Abbexa |
200 µl |
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Green One-Step qRT-PCR SuperMix |
abx098038-100l |
Abbexa |
100 µl |
EUR 737.5 |
Green One-Step qRT-PCR SuperMix |
abx098038-1ml |
Abbexa |
1 ml |
Ask for price |
Green One-Step qRT-PCR SuperMix |
abx098038-200l |
Abbexa |
200 µl |
EUR 1050 |
Probe One-Step qRT-PCR SuperMix |
abx098042-100l |
Abbexa |
100 µl |
EUR 850 |
Probe One-Step qRT-PCR SuperMix |
abx098042-1ml |
Abbexa |
1 ml |
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Probe One-Step qRT-PCR SuperMix |
abx098042-200l |
Abbexa |
200 µl |
EUR 1400 |
AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X) |
MB204-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 13 |
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 727.2 |
|
Two-Step RT-PCR SuperMix (15 kb) |
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 910.8 |
|
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-100l |
Abbexa |
100 µl |
EUR 887.5 |
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-1ml |
Abbexa |
1 ml |
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Two-Step RT-PCR SuperMix (12 kb) |
abx098025-200l |
Abbexa |
200 µl |
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Two-Step RT-PCR SuperMix (15 kb) |
abx098026-100l |
Abbexa |
100 µl |
EUR 1087.5 |
Two-Step RT-PCR SuperMix (15 kb) |
abx098026-1ml |
Abbexa |
1 ml |
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Two-Step RT-PCR SuperMix (15 kb) |
abx098026-200l |
Abbexa |
200 µl |
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All-in-One cDNA Synthesis SuperMix |
B24403 |
Bimake |
200 ractions |
EUR 547.2 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
B24408 |
Bimake |
1000 ractions |
EUR 1743.6 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
OmniPCR HS Supermix w Fluorescent dye |
MBA03-0100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 16 |
AmpFAST 2X PCR SuperMix (5-30 sec/kb; ≤6kb, 1X) |
MB202-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 11 |
OmniPCR FAST Supermix w Fluorescent dye |
MBA02-0100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 16 |
OmniPCR Long Supermix w Fluorescent dye |
MBA04-0100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 16 |
OnePCR Ultra Supermix w Fluorescent Dye |
MBS402026-100Reactions |
MyBiosource |
100Reactions |
EUR 180 |
OnePCR Ultra Supermix w Fluorescent Dye |
MBS402026-4Reactions |
MyBiosource |
4Reactions |
EUR 160 |
Fly First-Strand cDNA Synthesis SuperMix |
20-abx09885820ulSystems |
Abbexa |
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- 100 rxns × 20 ul Systems
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Fly First-Strand cDNA Synthesis SuperMix |
abx098858-100l |
Abbexa |
100 µl |
EUR 850 |
Fly First-Strand cDNA Synthesis SuperMix |
abx098858-1ml |
Abbexa |
1 ml |
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Fly First-Strand cDNA Synthesis SuperMix |
abx098858-200l |
Abbexa |
200 µl |
EUR 1037.5 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
abx098027-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 727.2 |
|
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098028-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 794.4 |
|
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098029-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 1028.4 |
|
One-Step RT-PCR SuperMix (+dye) (4 kb) |
abx098027-100l |
Abbexa |
100 µl |
EUR 875 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
abx098027-1ml |
Abbexa |
1 ml |
Ask for price |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
abx098027-200l |
Abbexa |
200 µl |
Ask for price |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098028-100l |
Abbexa |
100 µl |
EUR 950 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
abx098028-1ml |
Abbexa |
1 ml |
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A panel of synthetic scientific samples, 32 constructive and 30 adverse for SARS-CoV-2, had been analyzed to estimate the kappa concordance (κ) and the diagnostic efficiency. Variations among the many LoD values for the goal genes amplified by every equipment had been >1 log copies/response. The κ for the RT-qPCR kits was better than 0.9, whereas that for the RT-LAMP assays ranged from 0.75 to 0.93. The scientific efficiency of RT-qPCR kits confirmed 100% specificity and excessive sensitivity, though with variations in response to the gene analyzed. The E and N genes supplied better scientific sensitivity, whereas the RdRp gene elevated the scientific specificity. The RT-LAMP assays revealed a variable diagnostic efficiency. The knowledge supplied could be helpful to decide on essentially the most acceptable diagnostic check and will contribute to the institution of a consensus within the prognosis of SARS-CoV-2 in Argentina and the area.
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