Burkholderia mallei is the causative agent of glanders, a zoonosis listed by the World Group for Animal Well being as of obligatory notification. On this work, a comparability of three qPCR protocols was made, two of them based mostly on articles by different authors and one standardized in home, this final one aiming at a genomic area that doesn’t exist in different species of the Burkholderia genus. All qPCRs confirmed excessive effectivity and good repeatability. Nevertheless, reactions with Cq between 36 and 40 had been thought-about suspicious and unreliable, requiring better scientific standards to investigate the outcomes.

Knowledge on the optimization of an archaea-specific probe-based qPCR assay

Estimation of archaeal numbers by use of fluorescent DNA binding dyes is difficult, as a result of primers concentrating on the archaeal 16SrRNA genes readily additionally bind to bacterial 16S rRNA gene sequences, particularly when the relative abundance of micro organism is bigger than that of archaea. With the intention to enhance specificity, we optimized a fluorescent probe-based assay utilizing beforehand printed archaeal primers and probe. The assay was examined on genomic DNA of pure bacterial and archaeal cultures and optimized utilizing PCR amplicons of the archaeal pure cultures.
The used bacterial strains confirmed slight amplification utilizing the fluorescent dye assay, whereas all archaeal strains may very well be amplified with the archaea primers used. On account of variations in genome dimension and variety of 16S rRNA gene copies between the examined archaeal strains, the amplification degree different significantly between the strains.
 Validation of three qPCR for the detection of Burkholderia mallei in equine tissue samples
Due to this fact, we additionally examined the amplification utilizing PCR amplified fragments of the archaeal 16S rRNA genes. The checks with the archaeal 16S rRNA gene amplicons confirmed good amplification, though the amplification effectivity nonetheless different between archaeal strains. The qPCR assay was used to estimate the archaeal numbers in course of water of a multi-metal mine’s metallurgical plant [1] and might be utilized in comparable future microbiological evaluation included within the H2020 ITERAMS venture (Grant settlement# 730480).

Transcriptome analyses of urine RNA reveal tumor markers for human bladder most cancers: validated amplicons for RT-qPCR-based detection

Non-invasive scientific diagnostics of bladder most cancers is possible by way of a set of chemically distinct molecules together with macromolecular tumor markers comparable to polypeptides and nucleic acids. When it comes to tumor-related aberrant gene expression, RNA transcripts are the first indicator of tumor-specific gene expression as for polypeptides and their metabolic merchandise happen subsequently.
Thus, in case of bladder most cancers, urine RNA represents an early doubtlessly helpful diagnostic marker. Right here we describe a scientific deep transcriptome evaluation of consultant swimming pools of urine RNA collected from wholesome donors versus bladder most cancers sufferers in response to established SOPs. This evaluation revealed RNA marker candidates reflecting coding sequences, non-coding sequences, and round RNAs. Subsequent, we designed and validated PCR amplicons for a set of novel marker candidates and examined them in human bladder most cancers cell strains.
We recognized linear and round transcripts of the S100 Calcium Binding Protein 6 (S100A6) and translocation related membrane protein 1 (TRAM1) as extremely promising potential tumor markers. This work strongly suggests exploiting urine RNAs as diagnostic markers of bladder most cancers and it suggests particular novel markers. Additional, this research describes an entry into the tumor-biology of bladder most cancers and the event of gene-targeted therapeutic medication.

Analysis of RT-qPCR and Loop-Mediated Isothermal Amplification (LAMP) Assays for the Detection of SARS-CoV-2 in Argentina

Our purpose was to judge the analytical and scientific efficiency of the SARS-CoV-2 molecular detection kits utilized in Argentina. 9 real-time reverse-transcription polymerase chain response (RT-qPCR) and three reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assays had been evaluated utilizing the World Well being Group (WHO) beneficial check as reference methodology. A secondary commonplace calibrated for the E, N and RdRp genes towards the Pan American Well being Group-World Well being Group-Worldwide Normal was used to calculate the restrict of detection (LoD).

Green qPCR SuperMix UDG

20-abx098032
  • EUR 1135.00
  • EUR 453.00
  • EUR 662.00
  • 15 ml
  • 1 ml
  • 5 ml

Top Green qPCR SuperMix

20-abx098033
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix

20-abx098034
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Library Quantification qPCR SuperMix

20-abx098896
  • EUR 230.00
  • EUR 411.00
  • 1 ml
  • 5 ml

Pro QPCR SuperMix Kit - ROX premixed

K5056200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit - ROX premixed

K5056400 400 reactions
EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054200 200 reactions
EUR 408
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054400 400 reactions
EUR 594
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058200 200 reactions
EUR 211
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed

K5058400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Top Green qPCR SuperMix (+Dye II)

20-abx098890
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix (+Dye II)

20-abx098891
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 718

HiScript II Q RT SuperMix for qPCR

R222-01 100 rxn (20 μl/rxn)
EUR 235

HiScript II Q Select RT SuperMix for qPCR

R232-01 100 rxn (20 μl/rxn)
EUR 235

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

HiScript III RT SuperMix for qPCR (+g DNA wiper)

R323-01 100 rxn (20 μl/rxn)
EUR 293

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)

R233-01 100 rxn (20 μl/rxn)
EUR 244

PCR SuperMix

20-abx098887
  • EUR 272.00
  • EUR 578.00
  • 1 ml
  • 5 ml

electrophoresis power supply

EV245 ea
EUR 479

CyanRed Power Box

2305226 2unit
EUR 283
Description: i ndepent l y use for CyanRed Epi

Power Broth™

106 500 g
EUR 103

Power Broth™

117 500 ml
EUR 63

PCR SuperMix (-dye)

20-abx098002
  • EUR 411.00
  • EUR 189.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 5 ml

PCR SuperMix (+dye)

20-abx098003
  • EUR 411.00
  • EUR 189.00
  • EUR 6174.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 480 ml
  • 5 ml

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Plus PCR SuperMix

20-abx098888
  • EUR 244.00
  • EUR 467.00
  • 1 ml
  • 5 ml

Library Amplification SuperMix

20-abx098894
  • EUR 258.00
  • EUR 537.00
  • 1 ml
  • 5 ml

OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 89

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 141

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

Entrans qPCR Probe Kit

RK21209 40 RXN Ask for price

qPCR Control Template Kit

G929 100 rxns
EUR 148

Lentivirus qPCR Quantification Kit

K1471-100 100 Rxns
EUR 298

Retrovirus qPCR Quantification Kit

K1472-100 100 Rxns
EUR 318

QPCR Positive Control Kit

M1126-100
EUR 403

Four Source Power Supply 

EDK621 1 UNIT
EUR 684.23

Alkaline phosphatase Power AP

AZ140A 1MG
EUR 100.46

Alkaline phosphatase Power AP

AZ235A 1MG
EUR 116.12

12v vehicle power adapter

BSH100-A12 1 PC
EUR 429.53

Power Prime Broth™

112 500 g
EUR 111

Power Prime Broth™

122 500 ml
EUR 64

Power Prime-olate™

162 500 g
EUR 106

Power Prime-olate™

172 500 ml
EUR 68

COLLECTION CONTAINER 1L, EVA FILM

91-200-01 1/pk
EUR 80
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 2L, EVA FILM

91-200-02 1/pk
EUR 88
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 5L, EVA FILM

91-200-05 1/pk
EUR 99
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-10 1/pk
EUR 101
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-20 1/pk
EUR 110
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-36 1/pk
EUR 86
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-39 1/pk
EUR 89
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 1L, EVA FILM

91-200-42 1/pk
EUR 73
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 5L, EVA FILM

91-200-43 1/pk
EUR 151
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 10L, EVA FILM

91-200-45 1/pk
EUR 126
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 20L, EVA FILM

91-200-47 1/pk
EUR 132
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 50L, EVA FILM

91-200-48 1/pk
EUR 146
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 100L, EVA FILM

91-200-82 1/pk
EUR 279
Description: Flexible Containers; Bioprocess Bags and Parts

COLLECTION CONTAINER 200L, EVA FILM

91-200-83 1/pk
EUR 292
Description: Flexible Containers; Bioprocess Bags and Parts

T PCR SuperMix (-dye)

20-abx098005
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml

T PCR SuperMix (+dye)

20-abx098006
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml

EasyPfu PCR SuperMix (-dye)

20-abx098009
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml

FastPfu PCR SuperMix (-dye)

20-abx098010
  • EUR 244.00
  • EUR 495.00
  • 1 ml
  • 5 ml

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

Built in power supply for WSE-1165 Power AC 100-240V

WSE-650-659 1unit
EUR 726
Description: Assembl y power suppl y wi t h WSE-1165 such as bui l t -i n

Methylamp MS-qPCR Fast Kit

P-1028 200 Reactions
EUR 496.6
Description: fast delivery possible

Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
EUR 276.2

COLLECTION CONTAINER 500 ML, EVA FILM

91-200-41 1/pk
EUR 69
Description: Flexible Containers; Bioprocess Bags and Parts

electrophoresis power supply uk plug

EV245-UK ea
EUR 479

electrophoresis power supply 120 vac

EV245-US ea
EUR 479

Dual Source 150 Power Supply 

EDK620 1 UNIT
EUR 371.46

Power leads for HorizeBLOT-R

2322462 5unit
EUR 270

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 850

PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 411.00
  • EUR 189.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 5 ml

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
EUR 850

OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit

STA-859 200 assays
EUR 508
Description: OxiSelect Ferric Reducing Antioxidant Power (FRAP) Assay Kit is a quantitative assay for measuring the antioxidant potential within various samples. Following the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the sample, the kit colorimetric probe develops a blue color that is read colorimetrically at 540-600nm. The antioxidant potential of samples is determined based on an iron standard curve and results are calculated at Fe2+ equivalents (µM) or FRAP value.

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 3831.4

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 3831.4

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 3831.4

Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric)

K515-200
EUR 550

igScript RT-qPCR Kit - 50 Reactions

4513 1/EA
EUR 248

Coronavirus Rapid RT-qPCR Detection Kit

K1461-100 100 Rxns
EUR 551

Adeno-Associated Virus qPCR Quantification Kit

K1473-100 100 Rxns
EUR 348

Protein Eva-1 Homolog C (EVA1C) Antibody

20-abx302696
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

UNIVERSAL POWER SUPPLY FOR AXYGEN MOTOPET

MOTO-SP-PWR 1/pk
EUR 61
Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs

Power supply for LABeler Lab Printer

L9010-PS 1/pack
EUR 83.6
Description: Power supply for Labeler

ENDURO™ 250V Power Supply , 120V

LE0203-120 Ea
EUR 1603

ENDURO™ 250V Power Supply , 230V

LE0203-230 Ea
EUR 1603

ENDURO™ 300V Power Supply , 120V

LE0303-120 Ea
EUR 1198

myVolt™ Mini Power Supply,115V

LE1007 Ea
EUR 307

myVolt™ Mini Power Supply, 230V

LE1008 Ea
EUR 307

myVolt™ Touch power supply, 115V

LE1009 Ea
EUR 718

myVolt™ Touch power supply, 220V

LE1010 Ea
EUR 718

myVolt™ Mini power supply, 115V

BM0294 Ea
EUR 307

myVolt™ Mini power supply, 230V

BM0295 Ea
EUR 307

myVolt™ Touch power supply, 115V

BM0296 Ea
EUR 718

myVolt™ Touch power supply, 230V

BM0297 Ea
EUR 718

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055200 200 reactions
EUR 376
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055400 400 reactions
EUR 572
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Entrans qPCR Probe Set

RK21210 40 RXN Ask for price

Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 999

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1172

Regulatory RNA qPCR Profiler

RA950A-1 20 rxn
EUR 880

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-20rxns20ulSystems 20 rxns × 20 ul Systems
EUR 732

Green Two-Step qRT-PCR SuperMix

abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 648

Green One-Step qRT-PCR SuperMix

20-abx09803820ulSystems
  • EUR 495.00
  • EUR 746.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix

20-abx09804220ulSystems
  • EUR 592.00
  • EUR 1024.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Fly First-Strand cDNA Synthesis SuperMix

20-abx09885820ulSystems
  • EUR 718.00
  • EUR 578.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 456
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1453
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805

FRAP? (Ferric Reducing Antioxidant Power) Detection Kit (2 Plate)

K043-H1 2 x 96 well plate
EUR 496

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1132

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153

One.Step RT-qPCR Probe Kit (2X concentrated)

105-500 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Probe Kit (2X concentrated)

105-502 10 x 1,25 ml
EUR 1719

piggyBac qPCR copy number kit, 20 reactions  

PBC100A-1 20 reactions
EUR 331

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941

LABeler Lab Printer w/ 115V power supply

L9010 1/pack
EUR 187.3
Description: LABeler Hand Held Labeler

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941

LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles

RA910A-1 20 profiles
EUR 1108

SYBR Green qPCR Master Mix

HY-K0501 5 mL (500 rxns)
EUR 263

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 291

HotTaq Probe qPCR Mix (ROX)

BT11001 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)

BT11003 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix

BT11004 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)

BT11101 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (Capillary)

BT11103 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Universal Mix

BT11104 250rxn
EUR 138
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

TaqProbe 5X qPCR MasterMix-Multiplex

MasterMix-5PM 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-ROX

MasterMix-5R 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-iCycler

MasterMix-iC-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

KiloGreen 2X qPCR MasterMix-iCycler

MasterMix-KC 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-ROX

MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-iCycler

MasterMix-mC 4 x 1.25 ml for 500 reactions
EUR 151

BrightGreen miRNA qPCR MasterMix-ROX

MasterMix-mR 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-ROX

MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-iCycler

MasterMix-PC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-Multiplex

MasterMix-PM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-ROX

MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

BrightGreen 5X qPCR MasterMix-iCycler

MasterMix-5C 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 5X qPCR MasterMix-ROX

MasterMix-5P 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213
A panel of synthetic scientific samples, 32 constructive and 30 adverse for SARS-CoV-2, had been analyzed to estimate the kappa concordance (κ) and the diagnostic efficiency. Variations among the many LoD values for the goal genes amplified by every equipment had been >1 log copies/response. The κ for the RT-qPCR kits was better than 0.9, whereas that for the RT-LAMP assays ranged from 0.75 to 0.93. The scientific efficiency of RT-qPCR kits confirmed 100% specificity and excessive sensitivity, though with variations in response to the gene analyzed. The E and N genes supplied better scientific sensitivity, whereas the RdRp gene elevated the scientific specificity. The RT-LAMP assays revealed a variable diagnostic efficiency. The knowledge supplied could be helpful to decide on essentially the most acceptable diagnostic check and will contribute to the institution of a consensus within the prognosis of SARS-CoV-2 in Argentina and the area.

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