For 1 yr now, the world is present process a coronavirus disease-2019 (COVID-19) pandemic as a result of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Probably the most extensively used technique for COVID-19 prognosis is the detection of viral RNA Read more…
Burkholderia mallei is the causative agent of glanders, a zoonosis listed by the World Group for Animal Well being as of obligatory notification. On this work, a comparability of three qPCR protocols was made, two of them based mostly on Read more…
Diarrhea in mink kits is a significant reason for illness and mortality within the mink manufacturing. The etiology stays unknown in most outbreaks on account of a scarcity of diagnostic assays. Within the present examine we current an RT-qPCR methodology Read more…
To enrich RT-qPCR testing for analysis of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, many international locations have launched using fast antigen exams. As they typically show decrease real-life performances than anticipated, their right positioning as frontline screening continues Read more…
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is an accurate and convenient technique for quantifying expression levels of the target genes. Selection of the appropriate reference gene is of the vital importance for RT-qPCR analysis. Hippodamia variegata is one of the most important Read more…
The real-time reverse transcription-polymerase chain reaction (real-time RT-qPCR) has become a leading technique for the detection and quantification of arboviruses, including chikungunya, dengue, and zika viruses. In this study, an updated real-time RT-qPCR assay was designed and evaluated together with Read more…