Diarrhea in mink kits is a significant reason for illness and mortality within the mink manufacturing. The etiology stays unknown in most outbreaks on account of a scarcity of diagnostic assays. Within the present examine we current an RT-qPCR methodology to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sampled animals had been categorized primarily based on age and patoanatomical analysis as having pre-weaning diarrhea, diarrhea within the development interval or as having no macroscopic indicators of diarrhea.
Fecal samples had been analyzed for MiAstV with RT-qPCR, subsequent technology sequencing and electron microscopy in parallel. Mink astrovirus was detected with RT-qPCR in 92 out of 203 samples. This detection was confirmed by subsequent technology sequencing in a excessive proportion of samples (22/27), and by visualization of astrovirus particles with EM in among the samples. Mink astrovirus was extremely prevalent (68%) amongst kits within the outbreaks of pre-weaning diarrhea, specifically outbreaks from Could, whereas much less prevalent in outbreaks in June. Mink astrovirus was detected in outbreaks of diarrhea within the development interval, although in a a lot lesser extent than within the pre-weaning interval. The function of mink astrovirus within the diarrhea illness advanced of mink stay to be investigated, and for that objective this delicate and sturdy RT-qPCR is usually a useful instrument sooner or later.

Impact of disinfection brokers and quantification of doubtless viable Leptospira in contemporary water samples utilizing a extremely delicate integrity-qPCR assay

Leptospirosis is an rising worldwide zoonotic illness, however the common biology of the causative brokers continues to be poorly understood. People are an occasional host. The primary danger elements are water-associated publicity throughout skilled or leisure actions or throughout outbreaks in endemic areas. Detecting the presence of pathogenic micro organism in aquatic environments and their capability to withstand numerous inactivation processes are analysis fields that should be additional developed.
As well as, the strategies used for detecting and enumerating Leptospira nonetheless should be improved. We aimed to explain a brand new quantitative polymerase chain response coupled to propidium monoazide remedy (PMAqPCR) that targets not solely complete Leptospira but in addition discriminates pathogenic from non-pathogenic Leptospira whereas additionally addressing PCR inhibitors, a continuously encountered drawback when finding out environmental water. In a second step, the killing effectivity of Leptospira to totally different remedies was examined and PMAqPCR in comparison with culture-based enumeration.
 RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms
This offered details about the consequences of temperature, in addition to ultraviolet and chlorine disinfection, which are each associated to water remedy processes, specifically for the manufacturing of ingesting water, on the persistence of each saprophytic and pathogenic Leptospira. Lastly, PMAqPCR was used for the detection of Leptospira in freshwater samples for a proof-of-concept. In conclusion, our methodology might be used for routine freshwater monitoring and permits higher analysis of the presence of Leptospira, permitting analysis of the bacterial dynamics in a delegated space or evaluation of the efficacy of water disinfection processes.

luctuation of BCR-ABL1 qPCR IS stage past 0.1% IS after stopping tyrosine kinase inhibitor in persistent myeloid leukaemia sufferers with deep molecular response for not less than two years

Fluctuation of BCR-ABL1 real-time quantitative polymerase chain response in Worldwide Scale (qPCRIS) stage beneath main molecular response (MMR) (0.1%IS) is a identified phenomenon after stopping tyrosine kinase inhibitor (TKI) in persistent myeloid leukaemia (CML) sufferers who’re trying remedy free remission (TFR). We report right here 4 circumstances of fluctuation past MMR throughout conduct of a Malaysia Cease TKI Trial (MSIT) to look at the validity of the generally used relapse criterion – lack of MMR for one studying – aiming to supply proof in setting relapse standards for future CML sufferers who wish to try TFR.

Detection and identification of Kinetoplastids of zoonotic curiosity by HRM-qPCR evaluation in Canis lupus familiaris from Argentinean Mesopotamia

This work aimed to conduct a primary PCR-based strategy for differential analysis of kinetoplastidean infections in canine. Prognosis of Kinetoplastid infections in home animals is tough, since parasitemia is intermittent and indicators are nonspecific; it’s primarily primarily based on parasitological smears or focus strategies, which lack sensitivity and rely upon operator` experience. Canines are related reservoirs in transmission of Kinetoplastids; they perform as sentinels to detect energetic transmission cycles earlier than they contain people.
Trypanosoma cruzi, Trypanosoma evansi, and numerous species of Leishmania genus are multi-host parasites, able to parasitizing canine amongst an enormous variety of reservoirs. An algorithm primarily based on sequential Actual-Time PCR-Excessive Decision Melting (HRM) (qPCR-HRM) assays directed at 24S alpha ribosomal DNA, ITS1 and Hsp70 designed to tell apart amongst T. cruzi, T. rangeli, T. evansi and Leishmania spp. was examined in fourteen canine with suspicion of kinetoplastid illnesses.

Pro QPCR SuperMix Kit

K5053200 200 reactions
EUR 180

Pro QPCR SuperMix Kit

K5053400 400 reactions
EUR 326

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)

Q331-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (High ROX Premixed)

Q341-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)

Q431-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)

Q441-03 2,500 rxns (20 μl/rxn)
EUR 634

Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed)

RK21206 1 ml
EUR 328.5

Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed)

RK21207 1 ml
EUR 93

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)

EQ035 5mL
EUR 340

Eva QPCR SuperMix Kit

K5052200 200 reactions
EUR 211

Eva QPCR SuperMix Kit

K5052400 400 reactions
EUR 354

Fast Eva QPCR SuperMix Kit

K5052002 200 rxn
EUR 211

Power Eva QPCR SuperMix Kit

K5057200 200 reactions
EUR 180

Power Eva QPCR SuperMix Kit

K5057400 400 reactions
EUR 354

Probe qPCR SuperMix

20-abx098040
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  • 15 ml
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Green qPCR SuperMix

20-abx098031
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  • 15 ml
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Green qPCR SuperMix

abx098031-100l 100 µl
EUR 675

Green qPCR SuperMix

abx098031-200l 200 µl
EUR 912.5

Green qPCR SuperMix UDG

20-abx098032
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  • 15 ml
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Top Green qPCR SuperMix

20-abx098033
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Tip Green qPCR SuperMix

20-abx098034
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  • 15 ml
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Green qPCR SuperMix UDG

abx098032-100l 100 µl
EUR 687.5

Green qPCR SuperMix UDG

abx098032-200l 200 µl
EUR 912.5

Top Green qPCR SuperMix

abx098033-100l 100 µl
EUR 262.5

Top Green qPCR SuperMix

abx098033-200l 200 µl
EUR 487.5

Tip Green qPCR SuperMix

abx098034-100l 100 µl
EUR 262.5

Tip Green qPCR SuperMix

abx098034-200l 200 µl
EUR 437.5

EnTurbo™ probe qPCR SuperMix

EQ017 5mL
EUR 240

Library Quantification qPCR SuperMix

20-abx098896
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  • 1 ml
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Library Quantification qPCR SuperMix

abx098896-100l 100 µl
EUR 700

Library Quantification qPCR SuperMix

abx098896-200l 200 µl
EUR 962.5

Top Green qPCR SuperMix (+Dye II)

20-abx098890
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Tip Green qPCR SuperMix (+Dye II)

20-abx098891
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Top Green qPCR SuperMix (+Dye II)

abx098890-100l 100 µl
EUR 262.5

Top Green qPCR SuperMix (+Dye II)

abx098890-200l 200 µl
EUR 487.5

Tip Green qPCR SuperMix (+Dye II)

abx098891-100l 100 µl
EUR 262.5

Tip Green qPCR SuperMix (+Dye II)

abx098891-200l 200 µl
EUR 437.5

EnTurbo™ SYBR Color qPCR SuperMix

EQ036 5mL
EUR 340

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
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HyperScript RT SuperMix for qPCR

K1074-100 100 rxn (20 uL/rxn)
EUR 280
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HyperScript RT SuperMix for qPCR

K1074-50 50 rxn (20 uL/rxn)
EUR 150
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

cDNA Synthesis SuperMix for qPCR

abx098019-100l 100 µl
EUR 825

cDNA Synthesis SuperMix for qPCR

abx098019-1ml 1 ml
EUR 2387.5

cDNA Synthesis SuperMix for qPCR

abx098019-200l 200 µl
EUR 1000

1ml PrecisionPLUS qPCR Master Mix premixed with SYBRgreen

Z-PPLUS-SY-1ML 1ml
EUR 81
Description: Precision®PLUS

VAHTS Library Quantification Kit for Illumina (Low ROX Premixed)

NQ103 500 rxn
EUR 349.2

1ml Precision FAST qPCR Master Mix premixed with SYBRgreen

Z-PFAST-SY-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix premixed with SYBRgreen

Z-PFAST-SY-2ML 2ml
EUR 161
Description: Precision®FAST

HiScript II Q RT SuperMix for qPCR

R222-01 100 rxn (20 μl/rxn)
EUR 145

VAHTS Library Quantification Kit for Illumina (High ROX Premixed)

NQ104 500 rxn
EUR 349.2

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 861.6

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-100l 100 µl
EUR 1025

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-1ml 1 ml Ask for price

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-200l 200 µl Ask for price

HiScript II Q Select RT SuperMix for qPCR

R232-01 100 rxn (20 μl/rxn)
EUR 145

HiScript IV RT SuperMix for qPCR (+gDNA wiper)

R423-01 100rxns(20µl/rxn)
EUR 326

HiScript III RT SuperMix for qPCR (+gDNA wiper)

R323-01 100 rxn (20 μl/rxn)
EUR 217.5

Uni Cell to cDNA Synthesis SuperMix for qPCR

abx098860-100l 100 µl
EUR 1012.5

Uni Cell to cDNA Synthesis SuperMix for qPCR

abx098860-1ml 1 ml Ask for price

Uni Cell to cDNA Synthesis SuperMix for qPCR

abx098860-200l 200 µl Ask for price

HyperScript RT SuperMix for qPCR (with gDNA wiper)

K1174-100 100 rxns
EUR 304
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HyperScript RT SuperMix for qPCR (with gDNA wiper)

K1174-50 50 rxns
EUR 176
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method

HiScript II Q RT SuperMix for qPCR (+gDNA wiper)

R223-01 100rxns(20µl/rxn)
EUR 159.5

HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper)

R233-01 100 rxn (20 μl/rxn)
EUR 159.5

HiScript III All-in-one RT SuperMix Perfect for qPCR

R333-01 100 rxns (21 μl/rxn)
EUR 217.5

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055200 200 reactions
EUR 427

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055400 400 reactions
EUR 682

Fast QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055002 200 rxn
EUR 426

Fast QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055004 400 rxn
EUR 682

Animal Detection Probe qPCR Super PreMix

QV114-EN01 400 rxns
EUR 160

Animal Detection Probe qPCR Super PreMix

QV114-EN02 800 rxns
EUR 288

PCR SuperMix

20-abx098887
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Acryl/Bis 37.5:1 Premixed Powder, 200g

PR0604-200g each Ask for price

Acryl/Bis 37.5:1 Premixed Powder, 40g

PR0604-40g each Ask for price

2X PCR SuperMix

MB200-P100 100 rxns2 x 1.25 mL
EUR 11

Probe Direct SuperMix

abx461057-1096tests 10 × 96 tests Ask for price

Probe Direct SuperMix

abx461057-596tests 5 × 96 tests
EUR 2800

Probe Direct SuperMix

abx461057-96tests 96 tests
EUR 812.5

PCR SuperMix (-dye)

20-abx098002
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PCR SuperMix (+dye)

20-abx098003
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  • 15 ml
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  • 480 ml
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PCR SuperMix (-dye)

abx098002-100l 100 µl
EUR 237.5

PCR SuperMix (-dye)

abx098002-200l 200 µl
EUR 337.5

PCR SuperMix (+dye)

abx098003-100l 100 µl
EUR 237.5

PCR SuperMix (+dye)

abx098003-200l 200 µl
EUR 337.5

Plus PCR SuperMix

20-abx098888
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Evo? cDNA Supermix

M1168-100 each
EUR 457.2

Evo? cDNA Supermix

M1168-25 each
EUR 320.4

Plus PCR SuperMix

abx098888-100l 100 µl
EUR 300

Plus PCR SuperMix

abx098888-200l 200 µl
EUR 612.5

Novo? cDNA Supermix

M1169-100 each
EUR 529.2

Novo? cDNA Supermix

M1169-25 each
EUR 346.8

T PCR SuperMix (-dye)

20-abx098005
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T PCR SuperMix (+dye)

20-abx098006
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T PCR SuperMix (-dye)

abx098005-100l 100 µl
EUR 262.5

T PCR SuperMix (-dye)

abx098005-200l 200 µl
EUR 462.5

T PCR SuperMix (+dye)

abx098006-100l 100 µl
EUR 262.5

T PCR SuperMix (+dye)

abx098006-200l 200 µl
EUR 462.5

cDNA Synthesis SuperMix

20-abx09801420ulSystems
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cDNA Synthesis SuperMix

abx098014-100l 100 µl
EUR 725

cDNA Synthesis SuperMix

abx098014-1ml 1 ml Ask for price

cDNA Synthesis SuperMix

abx098014-200l 200 µl
EUR 812.5

AmpHS 2X PCR SuperMix

MB203-P100 100 rxns2 x 1.25 mL
EUR 11

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054200 200 reactions
EUR 468

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054400 400 reactions
EUR 711

EasyPfu PCR SuperMix (-dye)

20-abx098009
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  • 1 ml
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FastPfu PCR SuperMix (-dye)

20-abx098010
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  • 1 ml
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FastPfu PCR SuperMix (-dye)

abx098010-100l 100 µl
EUR 300

FastPfu PCR SuperMix (-dye)

abx098010-200l 200 µl
EUR 575

Library Amplification SuperMix

20-abx098894
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Library Amplification SuperMix

abx098894-100l 100 µl
EUR 737.5

Library Amplification SuperMix

abx098894-200l 200 µl
EUR 1137.5

OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 106.8

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 169.2

PCR SuperMix for PAGE (+dye)

20-abx098004
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PCR SuperMix for PAGE (+dye)

abx098004-100l 100 µl
EUR 237.5

PCR SuperMix for PAGE (+dye)

abx098004-200l 200 µl
EUR 337.5

Fast QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054002 200 rxn
EUR 468

Fast QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054004 400 rxn
EUR 711

Probe One-Step qRT-PCR SuperMix

20-abx09804220ulSystems
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Probe One-Step qRT-PCR SuperMix

abx098042-100l 100 µl
EUR 850

Probe One-Step qRT-PCR SuperMix

abx098042-1ml 1 ml Ask for price

Probe One-Step qRT-PCR SuperMix

abx098042-200l 200 µl
EUR 1400

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 202.8

OmniPCR Supermix w Fluorescent dye

MBA01-0100 100 rxns (2.5ml)
EUR 16

EnTurbo™ SYBR Green PCR SuperMix

EQ001 5mL
EUR 340

AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X)

MB204-P100 100 rxns2 x 1.25 mL
EUR 13

Green Two-Step qRT-PCR SuperMix

abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 777.6

Green One-Step qRT-PCR SuperMix

20-abx09803820ulSystems
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  • 100 rxns × 20 ul Systems
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Green Two-Step qRT-PCR SuperMix

abx098035-100l 100 µl
EUR 925

Green Two-Step qRT-PCR SuperMix

abx098035-1ml 1 ml Ask for price

Green Two-Step qRT-PCR SuperMix

abx098035-200l 200 µl Ask for price

Green One-Step qRT-PCR SuperMix

abx098038-100l 100 µl
EUR 737.5

Green One-Step qRT-PCR SuperMix

abx098038-1ml 1 ml Ask for price

Green One-Step qRT-PCR SuperMix

abx098038-200l 200 µl
EUR 1050

Two-Step RT-PCR SuperMix (12 kb)

abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 727.2

Two-Step RT-PCR SuperMix (15 kb)

abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)
EUR 910.8

Two-Step RT-PCR SuperMix (12 kb)

abx098025-100l 100 µl
EUR 887.5

Two-Step RT-PCR SuperMix (12 kb)

abx098025-1ml 1 ml Ask for price

Two-Step RT-PCR SuperMix (12 kb)

abx098025-200l 200 µl Ask for price

Two-Step RT-PCR SuperMix (15 kb)

abx098026-100l 100 µl
EUR 1087.5

Two-Step RT-PCR SuperMix (15 kb)

abx098026-1ml 1 ml Ask for price

Two-Step RT-PCR SuperMix (15 kb)

abx098026-200l 200 µl Ask for price

AmpFAST 2X PCR SuperMix (5-30 sec/kb; ≤6kb, 1X)

MB202-P100 100 rxns2 x 1.25 mL
EUR 11

ReadiUseâ„¢ Stayrightâ„¢ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*

45900 5 mL
EUR 227
Description: 3,3'-Diaminobenzidine (DAB) has been used as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

ReadiUseâ„¢ Stayrightâ„¢ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*

45900-5mL 5 mL
EUR 222
Description: 3,3'-Diaminobenzidine (DAB) has been used as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

ReadiUseâ„¢ Stayrightâ„¢ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*

45901 50 mL
EUR 455
Description: 3,3'-Diaminobenzidine (DAB) has been used as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

ReadiUseâ„¢ Stayrightâ„¢ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*

45901-50mL 50 mL
EUR 446
Description: 3,3'-Diaminobenzidine (DAB) has been used as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

OmniPCR HS Supermix w Fluorescent dye

MBA03-0100 100 rxns2 x 1.25 mL
EUR 16

OmniPCR FAST Supermix w Fluorescent dye

MBA02-0100 100 rxns2 x 1.25 mL
EUR 16

OmniPCR Long Supermix w Fluorescent dye

MBA04-0100 100 rxns2 x 1.25 mL
EUR 16

Probe One-Step qRT-PCR SuperMix (GC Rich)

20-abx09804120ulSystems
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  • 100 rxns × 20 ul Systems
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Probe One-Step qRT-PCR SuperMix (GC Rich)

abx098041-100l 100 µl
EUR 737.5

Probe One-Step qRT-PCR SuperMix (GC Rich)

abx098041-1ml 1 ml Ask for price

Probe One-Step qRT-PCR SuperMix (GC Rich)

abx098041-200l 200 µl
EUR 1050

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 727.2

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 794.4

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-200rxns20ulSystems 200 rxns × 20 ul Systems
EUR 1028.4

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-100l 100 µl
EUR 875

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-1ml 1 ml Ask for price

One-Step RT-PCR SuperMix (+dye) (4 kb)

abx098027-200l 200 µl Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-100l 100 µl
EUR 950

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-1ml 1 ml Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098028-200l 200 µl Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-100l 100 µl
EUR 1212.5

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-1ml 1 ml Ask for price

One-Step RT-PCR SuperMix (+dye) (8 kb)

abx098029-200l 200 µl Ask for price

Fly First-Strand cDNA Synthesis SuperMix

20-abx09885820ulSystems
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Fly First-Strand cDNA Synthesis SuperMix

abx098858-100l 100 µl
EUR 850

Fly First-Strand cDNA Synthesis SuperMix

abx098858-1ml 1 ml Ask for price

Fly First-Strand cDNA Synthesis SuperMix

abx098858-200l 200 µl
EUR 1037.5

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems 20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)
EUR 1062

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-100l 100 µl
EUR 1212.5

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-1ml 1 ml Ask for price

Green miRNA Two-Step qRT-PCR SuperMix

abx098036-200l 200 µl Ask for price

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-20rxns20ulSystems 20 rxns × 20 ul Systems
EUR 878.4

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-100l 100 µl
EUR 950

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-1ml 1 ml Ask for price

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-200l 200 µl Ask for price

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

20-abx098007
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  • 1 ml
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Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 961.2

Green One-Step qRT-PCR SuperMix (GC Rich)

20-abx09803920ulSystems
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  • 100 rxns × 20 ul Systems
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High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

abx098007-100l 100 µl
EUR 262.5

High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR)

abx098007-200l 200 µl
EUR 475

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-100l 100 µl
EUR 1125

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-1ml 1 ml Ask for price

Green Two-Step qRT-PCR SuperMix (GC Rich)

abx098037-200l 200 µl Ask for price

Green One-Step qRT-PCR SuperMix (GC Rich)

abx098039-100l 100 µl
EUR 450

Green One-Step qRT-PCR SuperMix (GC Rich)

abx098039-1ml 1 ml Ask for price

Green One-Step qRT-PCR SuperMix (GC Rich)

abx098039-200l 200 µl
EUR 1000

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 693.6

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-100l 100 µl
EUR 850

First-Strand cDNA Synthesis SuperMix for PCR

abx098018-1ml 1 ml Ask for price
A qPCR management of DNA integrity within the examined pattern, focused to the mammalian interphotoreceptor retinoid-binding protein (IRBP) gene fragment was included to the algorithm. T. evansi was detected in 4 canine and L. infantum in a single. Two of 5 qPCR optimistic circumstances had been smear unfavorable. Smear and T. evansi qPCR optimistic circumstances corresponded to animals that died regardless of being handled, indicating the affiliation of parasitemia with illness severity. This laboratory instrument will increase the potential of confirming outbreaks of kinetoplastid illnesses with zoonotic potential and determine the etiological brokers concerned.