Diarrhea in mink kits is a significant reason for illness and mortality within the mink manufacturing. The etiology stays unknown in most outbreaks on account of a scarcity of diagnostic assays. Within the present examine we current an RT-qPCR methodology to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sampled animals had been categorized primarily based on age and patoanatomical analysis as having pre-weaning diarrhea, diarrhea within the development interval or as having no macroscopic indicators of diarrhea.
Fecal samples had been analyzed for MiAstV with RT-qPCR, subsequent technology sequencing and electron microscopy in parallel. Mink astrovirus was detected with RT-qPCR in 92 out of 203 samples. This detection was confirmed by subsequent technology sequencing in a excessive proportion of samples (22/27), and by visualization of astrovirus particles with EM in among the samples. Mink astrovirus was extremely prevalent (68%) amongst kits within the outbreaks of pre-weaning diarrhea, specifically outbreaks from Could, whereas much less prevalent in outbreaks in June. Mink astrovirus was detected in outbreaks of diarrhea within the development interval, although in a a lot lesser extent than within the pre-weaning interval. The function of mink astrovirus within the diarrhea illness advanced of mink stay to be investigated, and for that objective this delicate and sturdy RT-qPCR is usually a useful instrument sooner or later.
Impact of disinfection brokers and quantification of doubtless viable Leptospira in contemporary water samples utilizing a extremely delicate integrity-qPCR assay
Leptospirosis is an rising worldwide zoonotic illness, however the common biology of the causative brokers continues to be poorly understood. People are an occasional host. The primary danger elements are water-associated publicity throughout skilled or leisure actions or throughout outbreaks in endemic areas. Detecting the presence of pathogenic micro organism in aquatic environments and their capability to withstand numerous inactivation processes are analysis fields that should be additional developed.
As well as, the strategies used for detecting and enumerating Leptospira nonetheless should be improved. We aimed to explain a brand new quantitative polymerase chain response coupled to propidium monoazide remedy (PMAqPCR) that targets not solely complete Leptospira but in addition discriminates pathogenic from non-pathogenic Leptospira whereas additionally addressing PCR inhibitors, a continuously encountered drawback when finding out environmental water. In a second step, the killing effectivity of Leptospira to totally different remedies was examined and PMAqPCR in comparison with culture-based enumeration.
This offered details about the consequences of temperature, in addition to ultraviolet and chlorine disinfection, which are each associated to water remedy processes, specifically for the manufacturing of ingesting water, on the persistence of each saprophytic and pathogenic Leptospira. Lastly, PMAqPCR was used for the detection of Leptospira in freshwater samples for a proof-of-concept. In conclusion, our methodology might be used for routine freshwater monitoring and permits higher analysis of the presence of Leptospira, permitting analysis of the bacterial dynamics in a delegated space or evaluation of the efficacy of water disinfection processes.
luctuation of BCR-ABL1 qPCR IS stage past 0.1% IS after stopping tyrosine kinase inhibitor in persistent myeloid leukaemia sufferers with deep molecular response for not less than two years
Fluctuation of BCR-ABL1 real-time quantitative polymerase chain response in Worldwide Scale (qPCRIS) stage beneath main molecular response (MMR) (0.1%IS) is a identified phenomenon after stopping tyrosine kinase inhibitor (TKI) in persistent myeloid leukaemia (CML) sufferers who’re trying remedy free remission (TFR). We report right here 4 circumstances of fluctuation past MMR throughout conduct of a Malaysia Cease TKI Trial (MSIT) to look at the validity of the generally used relapse criterion – lack of MMR for one studying – aiming to supply proof in setting relapse standards for future CML sufferers who wish to try TFR.
Detection and identification of Kinetoplastids of zoonotic curiosity by HRM-qPCR evaluation in Canis lupus familiaris from Argentinean Mesopotamia
This work aimed to conduct a primary PCR-based strategy for differential analysis of kinetoplastidean infections in canine. Prognosis of Kinetoplastid infections in home animals is tough, since parasitemia is intermittent and indicators are nonspecific; it’s primarily primarily based on parasitological smears or focus strategies, which lack sensitivity and rely upon operator` experience. Canines are related reservoirs in transmission of Kinetoplastids; they perform as sentinels to detect energetic transmission cycles earlier than they contain people.
Trypanosoma cruzi, Trypanosoma evansi, and numerous species of Leishmania genus are multi-host parasites, able to parasitizing canine amongst an enormous variety of reservoirs. An algorithm primarily based on sequential Actual-Time PCR-Excessive Decision Melting (HRM) (qPCR-HRM) assays directed at 24S alpha ribosomal DNA, ITS1 and Hsp70 designed to tell apart amongst T. cruzi, T. rangeli, T. evansi and Leishmania spp. was examined in fourteen canine with suspicion of kinetoplastid illnesses.
Eva QPCR SuperMix Kit |
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| K5052200 | Biochain | 200 reactions | EUR 211 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Eva QPCR SuperMix Kit |
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| K5052400 | Biochain | 400 reactions | EUR 320 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Power Eva QPCR SuperMix Kit |
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| K5057200 | Biochain | 200 reactions | EUR 188 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Power Eva QPCR SuperMix Kit |
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| K5057400 | Biochain | 400 reactions | EUR 320 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Green qPCR SuperMix UDG |
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| 20-abx098032 | Abbexa |
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Top Green qPCR SuperMix |
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| 20-abx098033 | Abbexa |
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Tip Green qPCR SuperMix |
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| 20-abx098034 | Abbexa |
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Library Quantification qPCR SuperMix |
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| 20-abx098896 | Abbexa |
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cDNA Synthesis SuperMix for qPCR |
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| 20-abx09801920ulSystems | Abbexa |
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Top Green qPCR SuperMix (+Dye II) |
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| 20-abx098890 | Abbexa |
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Tip Green qPCR SuperMix (+Dye II) |
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| 20-abx098891 | Abbexa |
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HotTaq Probe qPCR Mix (ROX) |
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| BT11001 | Bioatlas | 250rxn | EUR 109 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq EvaGreen qPCR Mix (ROX) |
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| BT11101 | Bioatlas | 250rxn | EUR 123 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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BrightGreen 5X qPCR MasterMix-ROX |
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| MasterMix-5R | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 213 |
KiloGreen 2X qPCR MasterMix-ROX |
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| MasterMix-KR | ABM | 4 x 1.25 ml - 500 reactions (20 ul) | EUR 140 |
BrightGreen miRNA qPCR MasterMix-ROX |
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| MasterMix-mR | ABM | 4 x 1.25 ml for 500 reactions | EUR 151 |
TaqProbe 2X qPCR MasterMix-ROX |
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| MasterMix-P | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
BrightGreen 2X qPCR MasterMix-ROX |
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| MasterMix-R | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
BrightGreen 2X qPCR MasterMix-ROX |
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| MasterMix-R-XL | ABM | 16 x 1.25 ml for 2000 reactions (20 ul) | EUR 376 |
TaqProbe 5X qPCR MasterMix-ROX |
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| MasterMix-5P | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 213 |
VAHTS Library Quantification Kit for Illumina (Low ROX Premixed) |
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| NQ103 | Vazyme | 500 rxn | EUR 291 |
VAHTS Library Quantification Kit for Illumina (High ROX Premixed) |
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| NQ104 | Vazyme | 500 rxn | EUR 291 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-50rxns20ulSystems | Abbexa | 50 rxns × 20 ul Systems | EUR 718 |
HiScript II Q RT SuperMix for qPCR |
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| R222-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 235 |
HotTaq Probe qPCR Mix (no ROX) |
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| BT11002 | Bioatlas | 250rxn | EUR 109 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq EvaGreen qPCR Mix (no ROX) |
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| BT11102 | Bioatlas | 250rxn | EUR 123 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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TaqProbe 5X qPCR MasterMix-Low ROX |
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| MasterMix-5PL | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 213 |
BrightGreen Express 2X qPCR MasterMix-ROX |
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| MasterMix-ER | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
KiloGreen 2X qPCR MasterMix-Low ROX |
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| MasterMix-KL | ABM | 4 x 1.25 ml - 500 reactions (20 ul) | EUR 140 |
BrightGreen 2X qPCR MasterMix-Low ROX |
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| MasterMix-LR | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
BrightGreen 2X qPCR MasterMix-Low ROX |
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| MasterMix-LR-XL | ABM | 16 x 1.25 ml for 2000 reactions (20 ul) | EUR 376 |
BrightGreen miRNA qPCR MasterMix-Low ROX |
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| MasterMix-mL | ABM | 4 x 1.25 ml for 500 reactions | EUR 151 |
TaqProbe 2X qPCR MasterMix-Low ROX |
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| MasterMix-PL | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
BrightGreen 5X qPCR MasterMix-Low ROX |
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| MasterMix-5L | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 213 |
HiScript II Q Select RT SuperMix for qPCR |
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| R232-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 235 |
SYBR Green qPCR Master Mix (High ROX) |
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| HY-K0521 | MedChemExpress | 1 mL (100 rxns) | EUR 113 |
SYBR Green qPCR Master Mix (Low ROX) |
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| HY-K0522 | MedChemExpress | 5 mL (500 rxns ) | EUR 257 |
SYBR Green qPCR Master Mix (No ROX) |
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| HY-K0523 | MedChemExpress | 5 mL (500 rxns ) | EUR 257 |
BrightGreen Express 2X qPCR MasterMix-Low ROX |
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| MasterMix-EL | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 140 |
ROX reference dye for qPCR, 1 mL |
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| 41110 | Lumiprobe | 1 mL | EUR 52 |
Accuris qMax Probe No Rox qPCR Mix |
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| PR2001-N-1000 | Benchmark Scientific | 1 PC | EUR 467.74 |
ROX reference dye for qPCR, 500 uL |
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| 31110 | Lumiprobe | 500 uL | EUR 46 |
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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| HS-100-QPCR | CORNING | 100/pk | EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
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PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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| HS-150-QPCR | CORNING | 100/pk | EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
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HiScript III RT SuperMix for qPCR (+g DNA wiper) |
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| R323-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 293 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
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| K5055200 | Biochain | 200 reactions | EUR 376 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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QCell-Pro One-Step qRT-PCR SuperMix Kit |
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| K5055400 | Biochain | 400 reactions | EUR 572 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
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| QPCR001-R | Bio Basic | 4X1.25ml | EUR 252.71 |
Genious 2X SYBRGREEN FAST qPCR MIX (Low ROX) |
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| RK21206 | Abclonal | 1 ml | Ask for price |
2x SYBR Green qPCR Master Mix (High ROX) |
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| B21402 | Bimake | 5 ml | EUR 224 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21403 | Bimake | 25 ml | EUR 856 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21702 | Bimake | 5 ml | EUR 224 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21703 | Bimake | 25 ml | EUR 856 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
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| QPCR002-L | Bio Basic | 4X1.25ml | EUR 252.71 |
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
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| RK21205 | Abclonal | 1 ml | Ask for price |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn |
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| AZ-2301 | Real Time Primers | 200 Rxn | EUR 179 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn |
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| AZ-2305 | Real Time Primers | 500 Rxn | EUR 320 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn |
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| AZ-2320 | Real Time Primers | 2000 Rxn | EUR 996 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn |
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| AZ-2325 | Real Time Primers | 5000 Rxn | EUR 2348 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn |
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| AZ-2401 | Real Time Primers | 200 Rxn | EUR 179 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn |
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| AZ-2405 | Real Time Primers | 500 Rxn | EUR 320 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn |
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| AZ-2420 | Real Time Primers | 2000 Rxn | EUR 996 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn |
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| AZ-2421 | Real Time Primers | 5000 Rxn | EUR 2348 |
HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
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| R233-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 244 |
T-Pro Plasmid Mini Kit (100) |
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| RB94-YPD100 | T-Pro Biotechnology | 100preps/Kit | EUR 161 |
T-Pro Plasmid Mini Kit (250) |
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| RB94-YPD250 | T-Pro Biotechnology | 250preps/Kit | EUR 222 |
T-Pro Plasmid Midi Kit (20) |
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| RB94-YPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 213 |
T-Pro Plasmid Maxi Kit (10) |
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| RB94-YPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 222 |
T-Pro BCA Protein Assay kit |
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| JB04-D001 | T-Pro Biotechnology | 500assay/KIT | EUR 204 |
Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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| W2601-1 | 101Bio | - | Ask for price |
Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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| W2601-5 | 101Bio | - | Ask for price |
T-Pro Genomic DNA Midi Kit (20) |
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| RB94-NGM020 | T-Pro Biotechnology | 20preps/kit | EUR 222 |
T-Pro Genomic DNA Mini Kit (100) |
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| RB94-NGS100 | T-Pro Biotechnology | 100preps/kit | EUR 213 |
T-Pro Bradford Protein Assay kit(1X) |
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| JB04-D002 | T-Pro Biotechnology | 500ml/BT | EUR 161 |
T-Pro Stacking Buffer |
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| JB03-C002 | T-Pro Biotechnology | 500ml/BT | EUR 152 |
T-Pro MycoClean spray |
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| JT90-R002 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
PCR SuperMix |
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| 20-abx098887 | Abbexa |
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T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K002M | T-Pro Biotechnology | 250ml*2/Kit | EUR 204 |
T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K002S | T-Pro Biotechnology | 100ml*2/Kit | EUR 152 |
T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K004M | T-Pro Biotechnology | 250ml*2/Kit | EUR 239 |
T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K004S | T-Pro Biotechnology | 100ml*2/Kit | EUR 170 |
Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
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| 31014 | Biotium | 2x1mL | EUR 228 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
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| 31014-1 | Biotium | 5x1mL | EUR 437 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
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| 31014-2 | Biotium | 50x1mL | EUR 3576 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
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| 31015-1 | Biotium | 5x1mL | EUR 437 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
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| 31015-2 | Biotium | 50x1mL | EUR 3576 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
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| 31042-1 | Biotium | 5x1mL | EUR 272 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
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| 31042-20mL | Biotium | 2x10mL | EUR 494 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
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| 31042-T | Biotium | 1mL | EUR 114 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
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| 9-31045 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
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| 9-31046 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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T-Pro Endotoxin Removal Plasmid Midi kit (25) |
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| RB94-EPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 248 |
T-Pro Endotoxin Removal Plasmid Maxi kit (10) |
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| RB94-EPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 265 |
T-Pro Plant Genomic DNA Midi Kit (20) |
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| RB94-PGM020 | T-Pro Biotechnology | 20preps/kit | EUR 222 |
T-Pro Plant Genomic DNA Mini Kit (100) |
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| RB94-PGS100 | T-Pro Biotechnology | 100preps/kit | EUR 222 |
T-Pro Gel/PCR DNA Purification Maxi Kit (10) |
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| RB94-NEL010 | T-Pro Biotechnology | 10preps/Kit | EUR 161 |
T-Pro Gel/PCR DNA Purification Mini Kit (100) |
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| RB94-NES100 | T-Pro Biotechnology | 100preps/Kit | EUR 161 |
T-Pro Gel/PCR DNA Purification Mini Kit (250) |
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| RB94-NES250 | T-Pro Biotechnology | 250preps/Kit | EUR 222 |
T-Pro EZ Gel Solution 8% |
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| JB02-B008M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 8% |
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| JB02-B008S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
T-Pro EZ Gel Solution 10% |
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| JB02-B010M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 10% |
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| JB02-B010S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
T-Pro EZ Gel Solution 12% |
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| JB02-B012M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 12% |
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| JB02-B012S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
T-Pro EZ Gel Solution 15% |
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| JB02-B015M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 15% |
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| JB02-B015S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
T-Pro Separating or Resolving Buffer |
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| JB03-C001 | T-Pro Biotechnology | 500ml/BT | EUR 152 |
T-Pro Transfer Blotting buffer (10X) |
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| JB08-H001 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
T-Pro Western Blot Stripping Reagent |
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| JB11-K002 | T-Pro Biotechnology | 500ml/BT | EUR 178 |
T-Pro Aqua EZ Clean (M) |
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| JT90-R001M | T-Pro Biotechnology | 500ml/BT | EUR 332 |
T-Pro Aqua EZ Clean (S) |
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| JT90-R001S | T-Pro Biotechnology | 100ml/BT | EUR 151 |
T-Pro EZ stain Gel solution |
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| JT90-R005M | T-Pro Biotechnology | 500ml/BT | EUR 187 |
T-Pro EZ stain Gel solution |
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| JT90-R005S | T-Pro Biotechnology | 100ml/BT | EUR 126 |
T-Pro P-Fect Transfection Reagent |
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| JT97-N005M | T-Pro Biotechnology | 1.0ml/vial | EUR 222 |
Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
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| 31014-T | Biotium | 1mL | EUR 134 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
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| 31015 | Biotium | 2x1mL | EUR 228 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
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| 31015-T | Biotium | 1mL | EUR 134 |
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Description: Minimum order quantity: 1 unit of 1mL |
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PCR SuperMix (-dye) |
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| 20-abx098002 | Abbexa |
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PCR SuperMix (+dye) |
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| 20-abx098003 | Abbexa |
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cDNA Synthesis SuperMix |
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| 20-abx09801420ulSystems | Abbexa |
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Plus PCR SuperMix |
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| 20-abx098888 | Abbexa |
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Library Amplification SuperMix |
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| 20-abx098894 | Abbexa |
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OneScriptcDNA Synthesis SuperMix |
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| G451 | ABM | 25 x 20 ul reactions | EUR 89 |
OneScriptcDNA Synthesis SuperMix |
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| G452 | ABM | 100 x 20 ul reactions | EUR 141 |
Evo? cDNA Supermix |
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| M1168-100 | Biovision | EUR 381 | |
Evo? cDNA Supermix |
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| M1168-25 | Biovision | EUR 267 | |
Novo? cDNA Supermix |
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| M1169-100 | Biovision | EUR 441 | |
Novo? cDNA Supermix |
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| M1169-25 | Biovision | EUR 289 | |
T-Pro BCA Protein Assay Reagent A |
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| JB04-D001A | T-Pro Biotechnology | 500ml/BT | EUR 170 |
T-Pro BCA Protein Assay Reagent B |
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| JB04-D001B | T-Pro Biotechnology | 12ml/BT | EUR 117 |
T-Pro Fast Blocking Buffer (in PBS) |
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| JK92-W001 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
T-Pro Fast Blocking Buffer (in TBS) |
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| JK92-W002 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
T-Pro Laemmli (SDS sample) Reagent (reducing 4X) |
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| JB06-F002 | T-Pro Biotechnology | 10ml/BT | EUR 126 |
T-Pro Tris-Glycine-SDS running buffer (10X) |
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| JB07-G001 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
T-Pro Tris-Glycine-Native running buffer (10X) |
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| JB07-G002 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
T-Pro Semi Dry Transfer Blotting buffer (10X) |
|||
| JB08-H002 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
T-Pro Protein Free Blocking Buffer (in PBS) |
|||
| JK92-W003 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
T-Pro Protein Free Blocking Buffer (in TBS) |
|||
| JK92-W004 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
T-Pro Nonliposomal Transfection Reagent I (NTR I) |
|||
| JT97-N001M | T-Pro Biotechnology | 1.0ml/vial | EUR 187 |
T-Pro Nonliposomal Transfection Reagent II (NTR II) |
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| JT97-N002M | T-Pro Biotechnology | 1.0ml/vial | EUR 204 |
T-Pro Nonliposomal Transfection Reagent III (NTR III) |
|||
| JT97-N006M | T-Pro Biotechnology | 1.0ml/vial | EUR 222 |
Entrans qPCR Probe Kit |
|||
| RK21209 | Abclonal | 40 RXN | Ask for price |
qPCR Control Template Kit |
|||
| G929 | ABM | 100 rxns | EUR 148 |
Lentivirus qPCR Quantification Kit |
|||
| K1471-100 | Biovision | 100 Rxns | EUR 298 |
Retrovirus qPCR Quantification Kit |
|||
| K1472-100 | Biovision | 100 Rxns | EUR 318 |
QPCR Positive Control Kit |
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| M1126-100 | Biovision | EUR 403 | |
6-ROX |
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| HY-D0053 | MedChemExpress | 5mg | EUR 187 |
5-ROX |
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| HY-D0784 | MedChemExpress | 50mg | EUR 601 |
5-ROX |
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| B8212-10 | ApexBio | 10 mg | EUR 284 |
5-ROX |
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| B8212-5 | ApexBio | 5 mg | EUR 195 |
5-ROX |
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| B8212-50 | ApexBio | 50 mg | EUR 773 |
Jade? QRT Kit-ROX |
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| M1178-100 | Biovision | EUR 370 | |
Taqman QRT Kit-ROX |
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| M1186-100 | Biovision | EUR 370 | |
T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X) |
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| JB06-F003 | T-Pro Biotechnology | 10ml/BT | EUR 126 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S210 | GeneOn | 100 rcs (2,5 ml) | EUR 138 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S210L | GeneOn | 500 rcs (12,5 ml) | EUR 523 |
T PCR SuperMix (-dye) |
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| 20-abx098005 | Abbexa |
|
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T PCR SuperMix (+dye) |
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| 20-abx098006 | Abbexa |
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EasyPfu PCR SuperMix (-dye) |
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| 20-abx098009 | Abbexa |
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FastPfu PCR SuperMix (-dye) |
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| 20-abx098010 | Abbexa |
|
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OneScriptPlus cDNA Synthesis SuperMix |
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| G453 | ABM | 25 x 20 ul reactions | EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G454 | ABM | 100 x 20 ul reactions | EUR 169 |
T-Pro Washing buffer in PBS and Tween-20 (10X) |
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| JB09-I003 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
T-Pro Washing buffer in TBS and Tween-20 (10X) |
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| JB09-I004 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS |
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| AP-STR-KIT-P | CORNING | 1/pk | EUR 721 |
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Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
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T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing |
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| JB09-I001 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing |
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| JB09-I002 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S170 | GeneOn | 100 rcs (2,5 ml) | EUR 138 |
qPCR Master Mix E3 (SafeLine: EvaGreen, dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S170L | GeneOn | 500 rcs (12,5 ml) | EUR 523 |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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| S175 | GeneOn | 100 rcs (2,5 ml) | EUR 138 |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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| S175L | GeneOn | 500 rcs (12,5 ml) | EUR 523 |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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| S215 | GeneOn | 100 rcs (2,5 ml) | EUR 159 |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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| S215L | GeneOn | 500 rcs (12,5 ml) | EUR 523 |
qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
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| S220 | GeneOn | 100 rcs (2,5 ml) | EUR 159 |
qPCR Master Mix DLP4 (SafeLine: dUTP, UNG, 500 nM ROX), packed in 1,25 ml vials |
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| S220L | GeneOn | 500 rcs (12,5 ml) | EUR 612 |
qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials |
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| S225 | GeneOn | 100 rcs (2,5 ml) | EUR 159 |
qPCR Master Mix DLP6 (SafeLine: dUTP, UNG, 100 nM ROX), packed in 1,25 ml vials |
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| S225L | GeneOn | 500 rcs (12,5 ml) | EUR 612 |
Methylamp MS-qPCR Fast Kit |
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| P-1028 | EpiGentek | 200 Reactions | EUR 496.6 |
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Description: fast delivery possible |
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SensiMix SYBR Hi-ROX Kit |
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| QT605-05 | Bioline | 500 rxn | Ask for price |
SensiMix SYBR Hi-ROX Kit |
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| QT605-20 | Bioline | 2000 rxn | Ask for price |
SensiMix SYBR Low-ROX Kit |
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| QT625-05 | Bioline | 500 rxn | Ask for price |
SensiMix SYBR Low-ROX Kit |
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| QT625-20 | Bioline | 2000 rxn | Ask for price |
SensiMix SYBR No-ROX Kit |
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| QT650-05 | Bioline | 500 rxn | Ask for price |
SensiMix SYBR No-ROX Kit |
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| QT650-20 | Bioline | 2000 rxn | Ask for price |
SensiFAST Genotyping Lo-Rox Kit |
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| BIO-34005 | Bioline | 500 Reactions | Ask for price |
SensiFAST Genotyping Hi-Rox Kit |
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| BIO-35005 | Bioline | 500 Reactions | Ask for price |
SensiFAST Probe Hi-ROX Kit |
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| BIO-82002/S | Bioline | Sample | Ask for price |
SensiFAST Probe Hi-ROX Kit |
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| BIO-82005 | Bioline | 500 rxns | Ask for price |
SensiFAST Probe Hi-ROX Kit |
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| BIO-82020 | Bioline | 2000 rxns | Ask for price |
SensiFAST Probe Lo-ROX Kit |
|||
| BIO-84002/S | Bioline | Sample | Ask for price |
SensiFAST Probe Lo-ROX Kit |
|||
| BIO-84005 | Bioline | 500 rxns | Ask for price |
SensiFAST Probe Lo-ROX Kit |
|||
| BIO-84020 | Bioline | 2000 rxns | Ask for price |
SensiFAST Probe No-ROX Kit |
|||
| BIO-86002/S | Bioline | Sample | Ask for price |
SensiFAST Probe No-ROX Kit |
|||
| BIO-86005 | Bioline | 500 rxns | Ask for price |
SensiFAST Probe No-ROX Kit |
|||
| BIO-86020 | Bioline | 2000 rxns | Ask for price |
SensiFAST SYBR Hi-ROX Kit |
|||
| BIO-92002/S | Bioline | Sample | Ask for price |
SensiFAST SYBR Hi-ROX Kit |
|||
| BIO-92005 | Bioline | 500 rxns | Ask for price |
SensiFAST SYBR Hi-ROX Kit |
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| BIO-92020 | Bioline | 2000 rxns | Ask for price |
SensiFAST SYBR Lo-ROX Kit |
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| BIO-94002/S | Bioline | Sample | Ask for price |
A qPCR management of DNA integrity within the examined pattern, focused to the mammalian interphotoreceptor retinoid-binding protein (IRBP) gene fragment was included to the algorithm. T. evansi was detected in 4 canine and L. infantum in a single. Two of 5 qPCR optimistic circumstances had been smear unfavorable. Smear and T. evansi qPCR optimistic circumstances corresponded to animals that died regardless of being handled, indicating the affiliation of parasitemia with illness severity. This laboratory instrument will increase the potential of confirming outbreaks of kinetoplastid illnesses with zoonotic potential and determine the etiological brokers concerned.
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