Diarrhea in mink kits is a significant reason for illness and mortality within the mink manufacturing. The etiology stays unknown in most outbreaks on account of a scarcity of diagnostic assays. Within the present examine we current an RT-qPCR methodology to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sampled animals had been categorized primarily based on age and patoanatomical analysis as having pre-weaning diarrhea, diarrhea within the development interval or as having no macroscopic indicators of diarrhea.
Fecal samples had been analyzed for MiAstV with RT-qPCR, subsequent technology sequencing and electron microscopy in parallel. Mink astrovirus was detected with RT-qPCR in 92 out of 203 samples. This detection was confirmed by subsequent technology sequencing in a excessive proportion of samples (22/27), and by visualization of astrovirus particles with EM in among the samples. Mink astrovirus was extremely prevalent (68%) amongst kits within the outbreaks of pre-weaning diarrhea, specifically outbreaks from Could, whereas much less prevalent in outbreaks in June. Mink astrovirus was detected in outbreaks of diarrhea within the development interval, although in a a lot lesser extent than within the pre-weaning interval. The function of mink astrovirus within the diarrhea illness advanced of mink stay to be investigated, and for that objective this delicate and sturdy RT-qPCR is usually a useful instrument sooner or later.
Impact of disinfection brokers and quantification of doubtless viable Leptospira in contemporary water samples utilizing a extremely delicate integrity-qPCR assay
Leptospirosis is an rising worldwide zoonotic illness, however the common biology of the causative brokers continues to be poorly understood. People are an occasional host. The primary danger elements are water-associated publicity throughout skilled or leisure actions or throughout outbreaks in endemic areas. Detecting the presence of pathogenic micro organism in aquatic environments and their capability to withstand numerous inactivation processes are analysis fields that should be additional developed.
As well as, the strategies used for detecting and enumerating Leptospira nonetheless should be improved. We aimed to explain a brand new quantitative polymerase chain response coupled to propidium monoazide remedy (PMAqPCR) that targets not solely complete Leptospira but in addition discriminates pathogenic from non-pathogenic Leptospira whereas additionally addressing PCR inhibitors, a continuously encountered drawback when finding out environmental water. In a second step, the killing effectivity of Leptospira to totally different remedies was examined and PMAqPCR in comparison with culture-based enumeration.
This offered details about the consequences of temperature, in addition to ultraviolet and chlorine disinfection, which are each associated to water remedy processes, specifically for the manufacturing of ingesting water, on the persistence of each saprophytic and pathogenic Leptospira. Lastly, PMAqPCR was used for the detection of Leptospira in freshwater samples for a proof-of-concept. In conclusion, our methodology might be used for routine freshwater monitoring and permits higher analysis of the presence of Leptospira, permitting analysis of the bacterial dynamics in a delegated space or evaluation of the efficacy of water disinfection processes.
luctuation of BCR-ABL1 qPCR IS stage past 0.1% IS after stopping tyrosine kinase inhibitor in persistent myeloid leukaemia sufferers with deep molecular response for not less than two years
Fluctuation of BCR-ABL1 real-time quantitative polymerase chain response in Worldwide Scale (qPCRIS) stage beneath main molecular response (MMR) (0.1%IS) is a identified phenomenon after stopping tyrosine kinase inhibitor (TKI) in persistent myeloid leukaemia (CML) sufferers who’re trying remedy free remission (TFR). We report right here 4 circumstances of fluctuation past MMR throughout conduct of a Malaysia Cease TKI Trial (MSIT) to look at the validity of the generally used relapse criterion – lack of MMR for one studying – aiming to supply proof in setting relapse standards for future CML sufferers who wish to try TFR.
Detection and identification of Kinetoplastids of zoonotic curiosity by HRM-qPCR evaluation in Canis lupus familiaris from Argentinean Mesopotamia
This work aimed to conduct a primary PCR-based strategy for differential analysis of kinetoplastidean infections in canine. Prognosis of Kinetoplastid infections in home animals is tough, since parasitemia is intermittent and indicators are nonspecific; it’s primarily primarily based on parasitological smears or focus strategies, which lack sensitivity and rely upon operator` experience. Canines are related reservoirs in transmission of Kinetoplastids; they perform as sentinels to detect energetic transmission cycles earlier than they contain people.
Trypanosoma cruzi, Trypanosoma evansi, and numerous species of Leishmania genus are multi-host parasites, able to parasitizing canine amongst an enormous variety of reservoirs. An algorithm primarily based on sequential Actual-Time PCR-Excessive Decision Melting (HRM) (qPCR-HRM) assays directed at 24S alpha ribosomal DNA, ITS1 and Hsp70 designed to tell apart amongst T. cruzi, T. rangeli, T. evansi and Leishmania spp. was examined in fourteen canine with suspicion of kinetoplastid illnesses.
Green qPCR SuperMix |
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| 20-abx098031 | Abbexa |
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Probe qPCR SuperMix |
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| 20-abx098040 | Abbexa |
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Power Eva QPCR SuperMix Kit |
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| K5057200 | Biochain | 200 reactions | EUR 225.6 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Power Eva QPCR SuperMix Kit |
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| K5057400 | Biochain | 400 reactions | EUR 384 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Fast Eva QPCR SuperMix Kit |
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| K5052002 | Biochain | 200 rxn | EUR 188 |
Green qPCR SuperMix UDG |
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| 20-abx098032 | Abbexa |
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Top Green qPCR SuperMix |
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| 20-abx098033 | Abbexa |
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Tip Green qPCR SuperMix |
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| 20-abx098034 | Abbexa |
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Library Quantification qPCR SuperMix |
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| 20-abx098896 | Abbexa |
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cDNA Synthesis SuperMix for qPCR |
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| 20-abx09801920ulSystems | Abbexa |
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VAHTS Library Quantification Kit for Illumina (Low ROX Premixed) |
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| NQ103 | Vazyme | 500 rxn | EUR 349.2 |
VAHTS Library Quantification Kit for Illumina (High ROX Premixed) |
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| NQ104 | Vazyme | 500 rxn | EUR 349.2 |
Top Green qPCR SuperMix (+Dye II) |
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| 20-abx098890 | Abbexa |
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Tip Green qPCR SuperMix (+Dye II) |
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| 20-abx098891 | Abbexa |
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HotTaq Probe qPCR Mix (ROX) |
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| BT11001 | Bioatlas | 250rxn | EUR 130.8 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq EvaGreen qPCR Mix (ROX) |
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| BT11101 | Bioatlas | 250rxn | EUR 147.6 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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TaqProbe 5X qPCR MasterMix-ROX |
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| MasterMix-5P | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 255.6 |
BrightGreen 5X qPCR MasterMix-ROX |
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| MasterMix-5R | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 255.6 |
KiloGreen 2X qPCR MasterMix-ROX |
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| MasterMix-KR | ABM | 4 x 1.25 ml - 500 reactions (20 ul) | EUR 168 |
BrightGreen miRNA qPCR MasterMix-ROX |
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| MasterMix-mR | ABM | 4 x 1.25 ml for 500 reactions | EUR 181.2 |
TaqProbe 2X qPCR MasterMix-ROX |
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| MasterMix-P | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
BrightGreen 2X qPCR MasterMix-ROX |
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| MasterMix-R | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
BrightGreen 2X qPCR MasterMix-ROX |
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| MasterMix-R-XL | ABM | 16 x 1.25 ml for 2000 reactions (20 ul) | EUR 451.2 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-50rxns20ulSystems | Abbexa | 50 rxns × 20 ul Systems | EUR 861.6 |
HiScript II Q RT SuperMix for qPCR |
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| R222-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 282 |
HotTaq Probe qPCR Mix (no ROX) |
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| BT11002 | Bioatlas | 250rxn | EUR 130.8 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq EvaGreen qPCR Mix (no ROX) |
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| BT11102 | Bioatlas | 250rxn | EUR 147.6 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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BrightGreen 5X qPCR MasterMix-Low ROX |
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| MasterMix-5L | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 255.6 |
TaqProbe 5X qPCR MasterMix-Low ROX |
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| MasterMix-5PL | ABM | 4 x 1.0 ml for 1000 reactions (20 ul) | EUR 255.6 |
BrightGreen Express 2X qPCR MasterMix-ROX |
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| MasterMix-ER | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
KiloGreen 2X qPCR MasterMix-Low ROX |
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| MasterMix-KL | ABM | 4 x 1.25 ml - 500 reactions (20 ul) | EUR 168 |
BrightGreen 2X qPCR MasterMix-Low ROX |
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| MasterMix-LR | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
BrightGreen 2X qPCR MasterMix-Low ROX |
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| MasterMix-LR-XL | ABM | 16 x 1.25 ml for 2000 reactions (20 ul) | EUR 451.2 |
BrightGreen miRNA qPCR MasterMix-Low ROX |
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| MasterMix-mL | ABM | 4 x 1.25 ml for 500 reactions | EUR 181.2 |
TaqProbe 2X qPCR MasterMix-Low ROX |
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| MasterMix-PL | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
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| K5055200 | Biochain | 200 reactions | EUR 451.2 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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QCell-Pro One-Step qRT-PCR SuperMix Kit |
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| K5055400 | Biochain | 400 reactions | EUR 686.4 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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QCell-Pro One-Step qRT-PCR SuperMix Kit |
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| K5055004 | Biochain | 400 rxn | EUR 609 |
HiScript II Q Select RT SuperMix for qPCR |
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| R232-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 282 |
QPCR Kit DNA FMV 35S pro |
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| MOL6434 | Scientific Laboratory Supplies | EACH | EUR 842.46 |
ROX reference dye for qPCR, 1 mL |
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| 41110 | Lumiprobe | 1 mL | EUR 62.4 |
ROX reference dye for qPCR, 500 uL |
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| 31110 | Lumiprobe | 500 uL | EUR 55.2 |
BrightGreen Express 2X qPCR MasterMix-Low ROX |
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| MasterMix-EL | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
SYBR Green qPCR Master Mix (High ROX) |
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| HY-K0521 | MedChemExpress | 1 mL (100 rxns) | EUR 135.6 |
SYBR Green qPCR Master Mix (Low ROX) |
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| HY-K0522 | MedChemExpress | 5 mL (500 rxns ) | EUR 308.4 |
SYBR Green qPCR Master Mix (No ROX) |
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| HY-K0523 | MedChemExpress | 5 mL (500 rxns ) | EUR 308.4 |
Accuris qMax Probe No Rox qPCR Mix |
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| PR2001-N-1000 | Benchmark Scientific | 1 PC | EUR 561.29 |
PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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| HS-100-QPCR | CORNING | 100/pk | EUR 381.6 |
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Description: Sealing Products; Sealing films - Axygen |
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PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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| HS-150-QPCR | CORNING | 100/pk | EUR 381.6 |
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Description: Sealing Products; Sealing films - Axygen |
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Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
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| K5055002 | Biochain | 200 rxn | EUR 380 |
HiScript III RT SuperMix for qPCR (+g DNA wiper) |
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| R323-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 351.6 |
QPCR Kit DNA FMV 35S pro Mmix |
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| MOL6436 | Scientific Laboratory Supplies | EACH | EUR 1071.6 |
2x SYBR Green qPCR Master Mix (High ROX) |
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| B21402 | Bimake | 5 ml | EUR 268.8 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21403 | Bimake | 25 ml | EUR 1027.2 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21702 | Bimake | 5 ml | EUR 268.8 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21703 | Bimake | 25 ml | EUR 1027.2 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
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| QPCR001-R | Bio Basic | 4X1.25ml | EUR 303.25 |
Genious 2X SYBRGREEN FAST qPCR MIX (Low ROX) |
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| RK21206 | Abclonal | 1 ml | Ask for price |
T-Pro BCA Protein Assay kit |
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| JB04-D001 | T-Pro Biotechnology | 500assay/KIT | EUR 244.8 |
T-Pro Plasmid Mini Kit (100) |
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| RB94-YPD100 | T-Pro Biotechnology | 100preps/Kit | EUR 193.2 |
T-Pro Plasmid Mini Kit (250) |
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| RB94-YPD250 | T-Pro Biotechnology | 250preps/Kit | EUR 266.4 |
T-Pro Plasmid Midi Kit (20) |
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| RB94-YPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 255.6 |
T-Pro Plasmid Maxi Kit (10) |
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| RB94-YPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 266.4 |
T-Pro Bradford Protein Assay kit(1X) |
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| JB04-D002 | T-Pro Biotechnology | 500ml/BT | EUR 193.2 |
T-Pro Genomic DNA Midi Kit (20) |
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| RB94-NGM020 | T-Pro Biotechnology | 20preps/kit | EUR 266.4 |
T-Pro Genomic DNA Mini Kit (100) |
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| RB94-NGS100 | T-Pro Biotechnology | 100preps/kit | EUR 255.6 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn |
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| AZ-2301 | Real Time Primers | 200 Rxn | EUR 214.8 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn |
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| AZ-2305 | Real Time Primers | 500 Rxn | EUR 384 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn |
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| AZ-2320 | Real Time Primers | 2000 Rxn | EUR 1195.2 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn |
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| AZ-2325 | Real Time Primers | 5000 Rxn | EUR 2817.6 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn |
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| AZ-2401 | Real Time Primers | 200 Rxn | EUR 214.8 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn |
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| AZ-2405 | Real Time Primers | 500 Rxn | EUR 384 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn |
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| AZ-2420 | Real Time Primers | 2000 Rxn | EUR 1195.2 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn |
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| AZ-2421 | Real Time Primers | 5000 Rxn | EUR 2817.6 |
Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
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| QPCR002-L | Bio Basic | 4X1.25ml | EUR 303.25 |
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
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| RK21205 | Abclonal | 1 ml | Ask for price |
PCR SuperMix |
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| 20-abx098887 | Abbexa |
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T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K002M | T-Pro Biotechnology | 250ml*2/Kit | EUR 244.8 |
T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K002S | T-Pro Biotechnology | 100ml*2/Kit | EUR 182.4 |
T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K004M | T-Pro Biotechnology | 250ml*2/Kit | EUR 286.8 |
T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K004S | T-Pro Biotechnology | 100ml*2/Kit | EUR 204 |
T-Pro Stacking Buffer |
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| JB03-C002 | T-Pro Biotechnology | 500ml/BT | EUR 182.4 |
T-Pro MycoClean spray |
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| JT90-R002 | T-Pro Biotechnology | 500ml/BT | EUR 172.8 |
HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
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| R233-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 292.8 |
T-Pro Endotoxin Removal Plasmid Midi kit (25) |
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| RB94-EPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 297.6 |
T-Pro Endotoxin Removal Plasmid Maxi kit (10) |
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| RB94-EPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 318 |
T-Pro Plant Genomic DNA Midi Kit (20) |
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| RB94-PGM020 | T-Pro Biotechnology | 20preps/kit | EUR 266.4 |
T-Pro Plant Genomic DNA Mini Kit (100) |
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| RB94-PGS100 | T-Pro Biotechnology | 100preps/kit | EUR 266.4 |
Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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| W2601-1 | 101Bio | - | Ask for price |
Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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| W2601-5 | 101Bio | - | Ask for price |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17282 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17283 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17284 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17285 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17286 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17287 | AAT Bioquest | 5 mL | EUR 308 |
T-Pro Gel/PCR DNA Purification Maxi Kit (10) |
|||
| RB94-NEL010 | T-Pro Biotechnology | 10preps/Kit | EUR 193.2 |
T-Pro Gel/PCR DNA Purification Mini Kit (100) |
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| RB94-NES100 | T-Pro Biotechnology | 100preps/Kit | EUR 193.2 |
T-Pro Gel/PCR DNA Purification Mini Kit (250) |
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| RB94-NES250 | T-Pro Biotechnology | 250preps/Kit | EUR 266.4 |
QPCR Kit QPCR Mastermix DNA |
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| MOL6354 | Scientific Laboratory Supplies | EACH | EUR 231.42 |
PCR SuperMix (-dye) |
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| 20-abx098002 | Abbexa |
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PCR SuperMix (+dye) |
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| 20-abx098003 | Abbexa |
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cDNA Synthesis SuperMix |
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| 20-abx09801420ulSystems | Abbexa |
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Plus PCR SuperMix |
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| 20-abx098888 | Abbexa |
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Library Amplification SuperMix |
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| 20-abx098894 | Abbexa |
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Evo? cDNA Supermix |
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| M1168-100 | Biovision | EUR 457.2 | |
Evo? cDNA Supermix |
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| M1168-25 | Biovision | EUR 320.4 | |
Novo? cDNA Supermix |
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| M1169-100 | Biovision | EUR 529.2 | |
Novo? cDNA Supermix |
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| M1169-25 | Biovision | EUR 346.8 | |
OneScriptcDNA Synthesis SuperMix |
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| G451 | ABM | 25 x 20 ul reactions | EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
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| G452 | ABM | 100 x 20 ul reactions | EUR 169.2 |
T-Pro EZ Gel Solution 8% |
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| JB02-B008M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 8% |
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| JB02-B008S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro EZ Gel Solution 10% |
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| JB02-B010M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 10% |
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| JB02-B010S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro EZ Gel Solution 12% |
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| JB02-B012M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 12% |
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| JB02-B012S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro EZ Gel Solution 15% |
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| JB02-B015M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 15% |
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| JB02-B015S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro Separating or Resolving Buffer |
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| JB03-C001 | T-Pro Biotechnology | 500ml/BT | EUR 182.4 |
T-Pro Transfer Blotting buffer (10X) |
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| JB08-H001 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
T-Pro Western Blot Stripping Reagent |
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| JB11-K002 | T-Pro Biotechnology | 500ml/BT | EUR 213.6 |
T-Pro Aqua EZ Clean (M) |
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| JT90-R001M | T-Pro Biotechnology | 500ml/BT | EUR 398.4 |
T-Pro Aqua EZ Clean (S) |
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| JT90-R001S | T-Pro Biotechnology | 100ml/BT | EUR 181.2 |
T-Pro EZ stain Gel solution |
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| JT90-R005M | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro EZ stain Gel solution |
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| JT90-R005S | T-Pro Biotechnology | 100ml/BT | EUR 151.2 |
T-Pro P-Fect Transfection Reagent |
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| JT97-N005M | T-Pro Biotechnology | 1.0ml/vial | EUR 266.4 |
Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
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| 31014 | Biotium | 2x1mL | EUR 273.6 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
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| 31014-1 | Biotium | 5x1mL | EUR 524.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
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| 31014-2 | Biotium | 50x1mL | EUR 4291.2 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
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| 31015-1 | Biotium | 5x1mL | EUR 524.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
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| 31015-2 | Biotium | 50x1mL | EUR 4291.2 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
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| 31042-1 | Biotium | 5x1mL | EUR 326.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
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| 31042-20mL | Biotium | 2x10mL | EUR 592.8 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
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| 31042-T | Biotium | 1mL | EUR 136.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
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| 9-31045 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
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| 9-31046 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17270 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
|||
| 17271 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
|||
| 17272 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
|||
| 17273 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
|||
| 17274 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
|||
| 17275 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17288 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
|||
| 17289 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
|||
| 17290 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
|||
| 17291 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
|||
| 17292 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17293 | AAT Bioquest | 5 mL | EUR 308 |
ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* |
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| 67004 | AAT Bioquest | 1 Plate | EUR 97 |
T-Pro BCA Protein Assay Reagent A |
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| JB04-D001A | T-Pro Biotechnology | 500ml/BT | EUR 204 |
T-Pro BCA Protein Assay Reagent B |
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| JB04-D001B | T-Pro Biotechnology | 12ml/BT | EUR 140.4 |
T-Pro Fast Blocking Buffer (in PBS) |
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| JK92-W001 | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro Fast Blocking Buffer (in TBS) |
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| JK92-W002 | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
Jade? QRT Kit-ROX |
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| M1178-100 | Biovision | EUR 444 | |
Taqman QRT Kit-ROX |
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| M1186-100 | Biovision | EUR 444 | |
T-Pro Laemmli (SDS sample) Reagent (reducing 4X) |
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| JB06-F002 | T-Pro Biotechnology | 10ml/BT | EUR 151.2 |
T-Pro Tris-Glycine-SDS running buffer (10X) |
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| JB07-G001 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
T-Pro Tris-Glycine-Native running buffer (10X) |
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| JB07-G002 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
T-Pro Semi Dry Transfer Blotting buffer (10X) |
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| JB08-H002 | T-Pro Biotechnology | 500ml/BT | EUR 172.8 |
T-Pro Protein Free Blocking Buffer (in PBS) |
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| JK92-W003 | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro Protein Free Blocking Buffer (in TBS) |
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| JK92-W004 | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro Nonliposomal Transfection Reagent I (NTR I) |
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| JT97-N001M | T-Pro Biotechnology | 1.0ml/vial | EUR 224.4 |
T-Pro Nonliposomal Transfection Reagent II (NTR II) |
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| JT97-N002M | T-Pro Biotechnology | 1.0ml/vial | EUR 244.8 |
T-Pro Nonliposomal Transfection Reagent III (NTR III) |
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| JT97-N006M | T-Pro Biotechnology | 1.0ml/vial | EUR 266.4 |
Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
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| 31014-T | Biotium | 1mL | EUR 160.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
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| 31015 | Biotium | 2x1mL | EUR 273.6 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
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| 31015-T | Biotium | 1mL | EUR 160.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17276 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17277 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17278 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17279 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17280 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17281 | AAT Bioquest | 5 mL | EUR 308 |
5-ROX |
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| B8212-10 | ApexBio | 10 mg | EUR 340.8 |
5-ROX |
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| B8212-5 | ApexBio | 5 mg | EUR 234 |
5-ROX |
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| B8212-50 | ApexBio | 50 mg | EUR 927.6 |
6-ROX |
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| HY-D0053 | MedChemExpress | 5mg | EUR 224.4 |
5-ROX |
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| HY-D0784 | MedChemExpress | 50mg | EUR 721.2 |
T PCR SuperMix (-dye) |
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| 20-abx098005 | Abbexa |
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T PCR SuperMix (+dye) |
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| 20-abx098006 | Abbexa |
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EasyPfu PCR SuperMix (-dye) |
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| 20-abx098009 | Abbexa |
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FastPfu PCR SuperMix (-dye) |
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| 20-abx098010 | Abbexa |
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OneScriptPlus cDNA Synthesis SuperMix |
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| G453 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G454 | ABM | 100 x 20 ul reactions | EUR 202.8 |
AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS |
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| AP-STR-KIT-P | CORNING | 1/pk | EUR 865.2 |
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Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
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T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X) |
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| JB06-F003 | T-Pro Biotechnology | 10ml/BT | EUR 151.2 |
Frit Kit |
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| FRIT-KIT | Next Advance | 1each | EUR 148.8 |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
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T-Pro Washing buffer in PBS and Tween-20 (10X) |
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| JB09-I003 | T-Pro Biotechnology | 500ml/BT | EUR 172.8 |
T-Pro Washing buffer in TBS and Tween-20 (10X) |
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| JB09-I004 | T-Pro Biotechnology | 500ml/BT | EUR 172.8 |
Column Packing Kit |
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| PACK-KIT | Next Advance | 1pack | EUR 1242 |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing |
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| JB09-I001 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing |
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| JB09-I002 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
SensiFAST Genotyping Lo-Rox Kit |
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| BIO-34005 | Bioline | 500 Reactions | Ask for price |
SensiFAST Genotyping Hi-Rox Kit |
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| BIO-35005 | Bioline | 500 Reactions | Ask for price |
SensiFAST Probe Hi-ROX Kit |
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| BIO-82002/S | Bioline | Sample | Ask for price |
SensiFAST Probe Hi-ROX Kit |
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| BIO-82005 | Bioline | 500 rxns | Ask for price |
SensiFAST Probe Hi-ROX Kit |
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| BIO-82020 | Bioline | 2000 rxns | Ask for price |
SensiFAST Probe Lo-ROX Kit |
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| BIO-84002/S | Bioline | Sample | Ask for price |
A qPCR management of DNA integrity within the examined pattern, focused to the mammalian interphotoreceptor retinoid-binding protein (IRBP) gene fragment was included to the algorithm. T. evansi was detected in 4 canine and L. infantum in a single. Two of 5 qPCR optimistic circumstances had been smear unfavorable. Smear and T. evansi qPCR optimistic circumstances corresponded to animals that died regardless of being handled, indicating the affiliation of parasitemia with illness severity. This laboratory instrument will increase the potential of confirming outbreaks of kinetoplastid illnesses with zoonotic potential and determine the etiological brokers concerned.
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