Diarrhea in mink kits is a significant reason for illness and mortality within the mink manufacturing. The etiology stays unknown in most outbreaks on account of a scarcity of diagnostic assays. Within the present examine we current an RT-qPCR methodology to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sampled animals had been categorized primarily based on age and patoanatomical analysis as having pre-weaning diarrhea, diarrhea within the development interval or as having no macroscopic indicators of diarrhea.
Fecal samples had been analyzed for MiAstV with RT-qPCR, subsequent technology sequencing and electron microscopy in parallel. Mink astrovirus was detected with RT-qPCR in 92 out of 203 samples. This detection was confirmed by subsequent technology sequencing in a excessive proportion of samples (22/27), and by visualization of astrovirus particles with EM in among the samples. Mink astrovirus was extremely prevalent (68%) amongst kits within the outbreaks of pre-weaning diarrhea, specifically outbreaks from Could, whereas much less prevalent in outbreaks in June. Mink astrovirus was detected in outbreaks of diarrhea within the development interval, although in a a lot lesser extent than within the pre-weaning interval. The function of mink astrovirus within the diarrhea illness advanced of mink stay to be investigated, and for that objective this delicate and sturdy RT-qPCR is usually a useful instrument sooner or later.
Impact of disinfection brokers and quantification of doubtless viable Leptospira in contemporary water samples utilizing a extremely delicate integrity-qPCR assay
Leptospirosis is an rising worldwide zoonotic illness, however the common biology of the causative brokers continues to be poorly understood. People are an occasional host. The primary danger elements are water-associated publicity throughout skilled or leisure actions or throughout outbreaks in endemic areas. Detecting the presence of pathogenic micro organism in aquatic environments and their capability to withstand numerous inactivation processes are analysis fields that should be additional developed.
As well as, the strategies used for detecting and enumerating Leptospira nonetheless should be improved. We aimed to explain a brand new quantitative polymerase chain response coupled to propidium monoazide remedy (PMAqPCR) that targets not solely complete Leptospira but in addition discriminates pathogenic from non-pathogenic Leptospira whereas additionally addressing PCR inhibitors, a continuously encountered drawback when finding out environmental water. In a second step, the killing effectivity of Leptospira to totally different remedies was examined and PMAqPCR in comparison with culture-based enumeration.
This offered details about the consequences of temperature, in addition to ultraviolet and chlorine disinfection, which are each associated to water remedy processes, specifically for the manufacturing of ingesting water, on the persistence of each saprophytic and pathogenic Leptospira. Lastly, PMAqPCR was used for the detection of Leptospira in freshwater samples for a proof-of-concept. In conclusion, our methodology might be used for routine freshwater monitoring and permits higher analysis of the presence of Leptospira, permitting analysis of the bacterial dynamics in a delegated space or evaluation of the efficacy of water disinfection processes.
luctuation of BCR-ABL1 qPCR IS stage past 0.1% IS after stopping tyrosine kinase inhibitor in persistent myeloid leukaemia sufferers with deep molecular response for not less than two years
Fluctuation of BCR-ABL1 real-time quantitative polymerase chain response in Worldwide Scale (qPCRIS) stage beneath main molecular response (MMR) (0.1%IS) is a identified phenomenon after stopping tyrosine kinase inhibitor (TKI) in persistent myeloid leukaemia (CML) sufferers who’re trying remedy free remission (TFR). We report right here 4 circumstances of fluctuation past MMR throughout conduct of a Malaysia Cease TKI Trial (MSIT) to look at the validity of the generally used relapse criterion – lack of MMR for one studying – aiming to supply proof in setting relapse standards for future CML sufferers who wish to try TFR.
Detection and identification of Kinetoplastids of zoonotic curiosity by HRM-qPCR evaluation in Canis lupus familiaris from Argentinean Mesopotamia
This work aimed to conduct a primary PCR-based strategy for differential analysis of kinetoplastidean infections in canine. Prognosis of Kinetoplastid infections in home animals is tough, since parasitemia is intermittent and indicators are nonspecific; it’s primarily primarily based on parasitological smears or focus strategies, which lack sensitivity and rely upon operator` experience. Canines are related reservoirs in transmission of Kinetoplastids; they perform as sentinels to detect energetic transmission cycles earlier than they contain people.
Trypanosoma cruzi, Trypanosoma evansi, and numerous species of Leishmania genus are multi-host parasites, able to parasitizing canine amongst an enormous variety of reservoirs. An algorithm primarily based on sequential Actual-Time PCR-Excessive Decision Melting (HRM) (qPCR-HRM) assays directed at 24S alpha ribosomal DNA, ITS1 and Hsp70 designed to tell apart amongst T. cruzi, T. rangeli, T. evansi and Leishmania spp. was examined in fourteen canine with suspicion of kinetoplastid illnesses.
EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed) |
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| EQ014-5mL | ELK Biotech | 5mL | EUR 80 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
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| EQ013 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
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| EQ013-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
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| EQ013-5mL | ELK Biotech | 5mL | EUR 80 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 120.55 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-02-500rxns | Vazyme | 500 rxns | EUR 124.83 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 543.5 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-03-2500rxns | Vazyme | 2,500 rxns | EUR 562.79 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 120.55 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-02-500rxns | Vazyme | 500 rxns | EUR 124.83 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 543.5 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-03-2500rxns | Vazyme | 2,500 rxns | EUR 562.79 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-02-500rxns | Vazyme | 500 rxns | EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-03-2500rxns | Vazyme | 2,500 rxns | EUR 656.51 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-02-500rxns | Vazyme | 500 rxns | EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 634 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-03-2500rxns | Vazyme | 2,500 rxns | EUR 656.51 |
Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed) |
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| RK21206 | Abclonal | 1 ml | EUR 76.3 |
Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed) |
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| RK21207 | Abclonal | 1 ml | EUR 21.26 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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| EQ035 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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| EQ035-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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| EQ035-5mL | ELK Biotech | 5mL | EUR 80 |
Probe qPCR SuperMix |
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| 20-abx098040 | Abbexa |
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Green qPCR SuperMix |
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| abx098031-100l | Abbexa | 100 µl | EUR 675 |
Green qPCR SuperMix |
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| 20-abx098031 | Abbexa |
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Green qPCR SuperMix |
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| abx098031-200l | Abbexa | 200 µl | EUR 912.5 |
Green qPCR SuperMix UDG |
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| abx098032-100l | Abbexa | 100 µl | EUR 687.5 |
Green qPCR SuperMix UDG |
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| 20-abx098032 | Abbexa |
|
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Green qPCR SuperMix UDG |
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| abx098032-200l | Abbexa | 200 µl | EUR 912.5 |
Top Green qPCR SuperMix |
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| abx098033-100l | Abbexa | 100 µl | EUR 262.5 |
Top Green qPCR SuperMix |
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| 20-abx098033 | Abbexa |
|
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Top Green qPCR SuperMix |
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| abx098033-200l | Abbexa | 200 µl | EUR 487.5 |
Tip Green qPCR SuperMix |
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| abx098034-100l | Abbexa | 100 µl | EUR 262.5 |
Tip Green qPCR SuperMix |
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| 20-abx098034 | Abbexa |
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Tip Green qPCR SuperMix |
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| abx098034-200l | Abbexa | 200 µl | EUR 437.5 |
EnTurbo™ probe qPCR SuperMix |
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| EQ017 | ELK Biotech | 5mL | EUR 240 |
EnTurbo™ probe qPCR SuperMix |
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| EQ017-25mL | ELK Biotech | 25mL | EUR 240 |
EnTurbo™ probe qPCR SuperMix |
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| EQ017-5mL | ELK Biotech | 5mL | EUR 78 |
Library Quantification qPCR SuperMix |
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| abx098896-100l | Abbexa | 100 µl | EUR 700 |
Library Quantification qPCR SuperMix |
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| abx098896-200l | Abbexa | 200 µl | EUR 962.5 |
Library Quantification qPCR SuperMix |
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| 20-abx098896 | Abbexa |
|
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Top Green qPCR SuperMix (+Dye II) |
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| abx098890-100l | Abbexa | 100 µl | EUR 262.5 |
Top Green qPCR SuperMix (+Dye II) |
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| 20-abx098890 | Abbexa |
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Top Green qPCR SuperMix (+Dye II) |
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| abx098890-200l | Abbexa | 200 µl | EUR 487.5 |
Tip Green qPCR SuperMix (+Dye II) |
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| abx098891-100l | Abbexa | 100 µl | EUR 262.5 |
Tip Green qPCR SuperMix (+Dye II) |
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| 20-abx098891 | Abbexa |
|
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Tip Green qPCR SuperMix (+Dye II) |
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| abx098891-200l | Abbexa | 200 µl | EUR 437.5 |
EnTurbo™ SYBR Color qPCR SuperMix |
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| EQ036 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix |
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| EQ036-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix |
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| EQ036-5mL | ELK Biotech | 5mL | EUR 80 |
cDNA Synthesis SuperMix for qPCR |
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| abx098019-100l | Abbexa | 100 µl | EUR 825 |
cDNA Synthesis SuperMix for qPCR |
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| 20-abx09801920ulSystems | Abbexa |
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cDNA Synthesis SuperMix for qPCR |
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| abx098019-1ml | Abbexa | 1 ml | EUR 2387.5 |
cDNA Synthesis SuperMix for qPCR |
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| abx098019-200l | Abbexa | 200 µl | EUR 1000 |
1ml PrecisionPLUS qPCR Master Mix premixed with SYBRgreen |
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| Z-PPLUS-SY-1ML | Novacyt Group | 1ml | EUR 81 |
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Description: Precision®PLUS |
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HyperScript™ RT SuperMix for qPCR |
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| K1074-100 | ApexBio | 100 rxn (20 uL/rxn) | EUR 296 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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HyperScript™ RT SuperMix for qPCR |
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| K1074-50 | ApexBio | 50 rxn (20 uL/rxn) | EUR 166.4 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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1ml Precision FAST qPCR Master Mix premixed with SYBRgreen |
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| Z-PFAST-SY-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST qPCR Master Mix premixed with SYBRgreen |
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| Z-PFAST-SY-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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HiScript II Q RT SuperMix for qPCR |
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| R222-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 145 |
VAHTS Library Quantification Kit for Illumina (Low ROX Premixed) |
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| NQ103 | Vazyme | 500 rxn | EUR 349.2 |
VAHTS Library Quantification Kit for Illumina (Low ROX Premixed) |
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| NQ103-500rxns | Vazyme | 500 rxns | EUR 181.21 |
VAHTS Library Quantification Kit for Illumina (High ROX Premixed) |
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| NQ104 | Vazyme | 500 rxn | EUR 349.2 |
VAHTS Library Quantification Kit for IIIumina (High ROX Premixed) |
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| NQ104-500rxns | Vazyme | 500 rxns | EUR 181.21 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-100l | Abbexa | 100 µl | EUR 1025 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-1ml | Abbexa | 1 ml | Ask for price |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-200l | Abbexa | 200 µl | Ask for price |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-50rxns20ulSystems | Abbexa | 50 rxns × 20 ul Systems | EUR 861.6 |
HiScript II Q Select RT SuperMix for qPCR |
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| R232-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 145 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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| R423-00-10rxns | Vazyme | 10 rxns | EUR 1.09 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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| R423-01 | Vazyme | 100rxns(20µl/rxn) | EUR 326 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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| R423-01-100rxns | Vazyme | 100 rxns | EUR 337.36 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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| abx098860-100l | Abbexa | 100 µl | EUR 1012.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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| abx098860-1ml | Abbexa | 1 ml | Ask for price |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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| abx098860-200l | Abbexa | 200 µl | Ask for price |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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| R323-00-10rxns | Vazyme | 10 rxns | EUR 1.09 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
|||
| R323-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 217.5 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
|||
| R323-01-100rxns | Vazyme | 100 rxns | EUR 225.22 |
HiScript II Q RT SuperMix for qPCR (+gDNA wiper) |
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| R223-01 | Vazyme | 100rxns(20µl/rxn) | EUR 159.5 |
HyperScript™ RT SuperMix for qPCR (with gDNA wiper) |
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| K1174-100 | ApexBio | 100 rxns | EUR 320 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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HyperScript™ RT SuperMix for qPCR (with gDNA wiper) |
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| K1174-50 | ApexBio | 50 rxns | EUR 192 |
|
Description: Reverse Transcription & PCR|Reverse Transcription |
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HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) |
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| R233-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 159.5 |
Pro-QPCR-SuperMix-Kit---ROX-premixed |
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| K5056200 | Biochain | 200 reactions | EUR 126 |
Pro-QPCR-SuperMix-Kit---ROX-premixed |
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| K5056400 | Biochain | 400 reactions | EUR 228.2 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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| R333-00-10rxns | Vazyme | 10 rxns | EUR 1.09 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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| R333-01 | Vazyme | 100 rxns (21 μl/rxn) | EUR 217.5 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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| R333-01-100rxns | Vazyme | 100 rxns | EUR 225.22 |
Fast-Pro-QPCR-SuperMix-Kit---ROX-premixed |
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| K5058200 | Biochain | 200 reactions | EUR 147.7 |
Fast-Pro-QPCR-SuperMix-Kit---ROX-premixed |
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| K5058400 | Biochain | 400 reactions | EUR 247.8 |
Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus) |
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| 11141ES10 | Yeasen Biotechnology | 10T | EUR 25 |
Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus) |
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| 11141ES60 | Yeasen Biotechnology | 100T | EUR 230 |
Animal Detection Probe qPCR Super PreMix |
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| QV114-EN01 | Vazyme | 400 rxns | EUR 160 |
Animal Detection Probe qPCR Super PreMix |
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| QV114-EN01-400rxns | Vazyme | 400 rxns | EUR 165.68 |
Animal Detection Probe qPCR Super PreMix |
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| QV114-EN02 | Vazyme | 800 rxns | EUR 288 |
Animal Detection Probe qPCR Super PreMix |
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| QV114-EN02-800rxns | Vazyme | 800 rxns | EUR 298.22 |
PCR SuperMix |
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| 20-abx098887 | Abbexa |
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Acryl/Bis 37.5:1 Premixed Powder, 200g |
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| PR0604-200g | Vivantis | each | Ask for price |
Acryl/Bis 37.5:1 Premixed Powder, 40g |
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| PR0604-40g | Vivantis | each | Ask for price |
2X PCR SuperMix |
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| MB200-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
2X PCR SuperMix |
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| MBS402025-100Reactions | MyBiosource | 100Reactions | EUR 175 |
2X PCR SuperMix |
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| MBS402025-4Reactions | MyBiosource | 4Reactions | EUR 160 |
Probe Direct SuperMix |
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| abx461057-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Probe Direct SuperMix |
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| abx461057-596tests | Abbexa | 5 × 96 tests | EUR 2800 |
Probe Direct SuperMix |
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| abx461057-96tests | Abbexa | 96 tests | EUR 812.5 |
PCR SuperMix (-dye) |
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| abx098002-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix (-dye) |
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| 20-abx098002 | Abbexa |
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PCR SuperMix (-dye) |
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| abx098002-200l | Abbexa | 200 µl | EUR 337.5 |
PCR SuperMix (+dye) |
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| abx098003-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix (+dye) |
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| 20-abx098003 | Abbexa |
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PCR SuperMix (+dye) |
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| abx098003-200l | Abbexa | 200 µl | EUR 337.5 |
Plus PCR SuperMix |
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| abx098888-100l | Abbexa | 100 µl | EUR 300 |
Plus PCR SuperMix |
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| abx098888-200l | Abbexa | 200 µl | EUR 612.5 |
Plus PCR SuperMix |
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| 20-abx098888 | Abbexa |
|
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Evo? cDNA Supermix |
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| M1168-100 | Biovision | each | EUR 457.2 |
Evo? cDNA Supermix |
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| M1168-25 | Biovision | each | EUR 320.4 |
Novo? cDNA Supermix |
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| M1169-100 | Biovision | each | EUR 529.2 |
Novo? cDNA Supermix |
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| M1169-25 | Biovision | each | EUR 346.8 |
cDNA Synthesis SuperMix |
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| abx098014-100l | Abbexa | 100 µl | EUR 725 |
cDNA Synthesis SuperMix |
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| 20-abx09801420ulSystems | Abbexa |
|
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cDNA Synthesis SuperMix |
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| abx098014-1ml | Abbexa | 1 ml | Ask for price |
cDNA Synthesis SuperMix |
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| abx098014-200l | Abbexa | 200 µl | EUR 812.5 |
T PCR SuperMix (-dye) |
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| abx098005-100l | Abbexa | 100 µl | EUR 262.5 |
T PCR SuperMix (-dye) |
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| abx098005-200l | Abbexa | 200 µl | EUR 462.5 |
T PCR SuperMix (-dye) |
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| 20-abx098005 | Abbexa |
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T PCR SuperMix (+dye) |
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| abx098006-100l | Abbexa | 100 µl | EUR 262.5 |
T PCR SuperMix (+dye) |
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| abx098006-200l | Abbexa | 200 µl | EUR 462.5 |
T PCR SuperMix (+dye) |
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| 20-abx098006 | Abbexa |
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AmpHS 2X PCR SuperMix |
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| MB203-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
qPCR PreMix Kit |
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| MB1001-2 | BosterBio | 1 Kit | EUR 276 |
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Description: qPCR PreMix is a kit for providing accurate data from your various samples speedily. There are no additional mixing steps because it already prepared and dried for your convenient. This product makes you monitor your gene amplification of Ct in every steps of amplified florescent cycle because we add “probe” which include florescence oligo-nucleotide and primers. |
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EasyPfu PCR SuperMix (-dye) |
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| 20-abx098009 | Abbexa |
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FastPfu PCR SuperMix (-dye) |
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| abx098010-100l | Abbexa | 100 µl | EUR 300 |
FastPfu PCR SuperMix (-dye) |
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| abx098010-200l | Abbexa | 200 µl | EUR 575 |
FastPfu PCR SuperMix (-dye) |
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| 20-abx098010 | Abbexa |
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Library Amplification SuperMix |
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| abx098894-100l | Abbexa | 100 µl | EUR 737.5 |
Library Amplification SuperMix |
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| abx098894-200l | Abbexa | 200 µl | EUR 1137.5 |
Library Amplification SuperMix |
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| 20-abx098894 | Abbexa |
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Premixed thermal fluid ready to use, box of 5 litres |
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| CB-1002322 | Phoenix instrument | each | EUR 81 |
OneScriptcDNA Synthesis SuperMix |
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| G451 | ABM | 25 x 20 ul reactions | EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
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| G452 | ABM | 100 x 20 ul reactions | EUR 169.2 |
PCR SuperMix for PAGE (+dye) |
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| abx098004-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix for PAGE (+dye) |
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| 20-abx098004 | Abbexa |
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PCR SuperMix for PAGE (+dye) |
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| abx098004-200l | Abbexa | 200 µl | EUR 337.5 |
Probe One-Step qRT-PCR SuperMix |
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| abx098042-100l | Abbexa | 100 µl | EUR 850 |
Probe One-Step qRT-PCR SuperMix |
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| 20-abx09804220ulSystems | Abbexa |
|
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Probe One-Step qRT-PCR SuperMix |
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| abx098042-1ml | Abbexa | 1 ml | Ask for price |
Probe One-Step qRT-PCR SuperMix |
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| abx098042-200l | Abbexa | 200 µl | EUR 1400 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G453 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G454 | ABM | 100 x 20 ul reactions | EUR 202.8 |
OmniPCR Supermix w Fluorescent dye |
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| MBA01-0100 | Bio-Helix | 100 rxns (2.5ml) | EUR 16 |
EnTurbo™ SYBR Green PCR SuperMix |
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| EQ001 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix |
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| EQ001-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix |
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| EQ001-5mL | ELK Biotech | 5mL | EUR 80 |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-100l | Abbexa | 100 µl | EUR 925 |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-1ml | Abbexa | 1 ml | Ask for price |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-200l | Abbexa | 200 µl | Ask for price |
Green Two-Step qRT-PCR SuperMix |
|||
| abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems | Abbexa | 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) | EUR 777.6 |
Green One-Step qRT-PCR SuperMix |
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| abx098038-100l | Abbexa | 100 µl | EUR 737.5 |
Green One-Step qRT-PCR SuperMix |
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| 20-abx09803820ulSystems | Abbexa |
|
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Green One-Step qRT-PCR SuperMix |
|||
| abx098038-1ml | Abbexa | 1 ml | Ask for price |
Green One-Step qRT-PCR SuperMix |
|||
| abx098038-200l | Abbexa | 200 µl | EUR 1050 |
AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X) |
|||
| MB204-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 13 |
ReadiUseâ„¢ Stayrightâ„¢ Purple *HRP Chromogen Premixed with Hydrogen Peroxide* |
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| 45900 | AAT Bioquest | 5 mL | EUR 227 |
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Description: 3,3'-Diaminobenzidine (DAB) has been used as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications. |
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ReadiUseâ„¢ Stayrightâ„¢ Purple *HRP Chromogen Premixed with Hydrogen Peroxide* |
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| 45900-5mL | AAT Bioquest | 5 mL | EUR 222 |
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Description: 3,3'-Diaminobenzidine (DAB) has been used as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications. |
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ReadiUseâ„¢ Stayrightâ„¢ Purple *HRP Chromogen Premixed with Hydrogen Peroxide* |
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| 45901 | AAT Bioquest | 50 mL | EUR 455 |
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Description: 3,3'-Diaminobenzidine (DAB) has been used as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications. |
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ReadiUseâ„¢ Stayrightâ„¢ Purple *HRP Chromogen Premixed with Hydrogen Peroxide* |
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| 45901-50mL | AAT Bioquest | 50 mL | EUR 446 |
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Description: 3,3'-Diaminobenzidine (DAB) has been used as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications. |
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Two-Step RT-PCR SuperMix (12 kb) |
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| abx098025-100l | Abbexa | 100 µl | EUR 887.5 |
Two-Step RT-PCR SuperMix (12 kb) |
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| abx098025-1ml | Abbexa | 1 ml | Ask for price |
Two-Step RT-PCR SuperMix (12 kb) |
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| abx098025-200l | Abbexa | 200 µl | Ask for price |
Two-Step RT-PCR SuperMix (12 kb) |
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| abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems | Abbexa | 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) | EUR 727.2 |
Two-Step RT-PCR SuperMix (15 kb) |
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| abx098026-100l | Abbexa | 100 µl | EUR 1087.5 |
Two-Step RT-PCR SuperMix (15 kb) |
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| abx098026-1ml | Abbexa | 1 ml | Ask for price |
Two-Step RT-PCR SuperMix (15 kb) |
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| abx098026-200l | Abbexa | 200 µl | Ask for price |
Two-Step RT-PCR SuperMix (15 kb) |
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| abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems | Abbexa | 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) | EUR 910.8 |
All-in-One cDNA Synthesis SuperMix |
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| B24403 | Bimake | 200 ractions | EUR 547.2 |
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Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
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All-in-One cDNA Synthesis SuperMix |
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| B24408 | Bimake | 1000 ractions | EUR 1743.6 |
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Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
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OmniPCR HS Supermix w Fluorescent dye |
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| MBA03-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
AmpFAST 2X PCR SuperMix (5-30 sec/kb; ≤6kb, 1X) |
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| MB202-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
OmniPCR FAST Supermix w Fluorescent dye |
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| MBA02-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
OmniPCR Long Supermix w Fluorescent dye |
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| MBA04-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
OnePCR Ultra Supermix w Fluorescent Dye |
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| MBS402026-100Reactions | MyBiosource | 100Reactions | EUR 180 |
OnePCR Ultra Supermix w Fluorescent Dye |
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| MBS402026-4Reactions | MyBiosource | 4Reactions | EUR 160 |
Fly First-Strand cDNA Synthesis SuperMix |
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| abx098858-100l | Abbexa | 100 µl | EUR 850 |
Fly First-Strand cDNA Synthesis SuperMix |
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| 20-abx09885820ulSystems | Abbexa |
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Fly First-Strand cDNA Synthesis SuperMix |
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| abx098858-1ml | Abbexa | 1 ml | Ask for price |
Fly First-Strand cDNA Synthesis SuperMix |
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| abx098858-200l | Abbexa | 200 µl | EUR 1037.5 |
Probe One-Step qRT-PCR SuperMix (GC Rich) |
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| abx098041-100l | Abbexa | 100 µl | EUR 737.5 |
Probe One-Step qRT-PCR SuperMix (GC Rich) |
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| 20-abx09804120ulSystems | Abbexa |
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Probe One-Step qRT-PCR SuperMix (GC Rich) |
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| abx098041-1ml | Abbexa | 1 ml | Ask for price |
Probe One-Step qRT-PCR SuperMix (GC Rich) |
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| abx098041-200l | Abbexa | 200 µl | EUR 1050 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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| abx098027-100l | Abbexa | 100 µl | EUR 875 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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| abx098027-1ml | Abbexa | 1 ml | Ask for price |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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| abx098027-200l | Abbexa | 200 µl | Ask for price |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
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| abx098027-200rxns20ulSystems | Abbexa | 200 rxns × 20 ul Systems | EUR 727.2 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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| abx098028-100l | Abbexa | 100 µl | EUR 950 |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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| abx098028-1ml | Abbexa | 1 ml | Ask for price |
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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| abx098028-200l | Abbexa | 200 µl | Ask for price |
A qPCR management of DNA integrity within the examined pattern, focused to the mammalian interphotoreceptor retinoid-binding protein (IRBP) gene fragment was included to the algorithm. T. evansi was detected in 4 canine and L. infantum in a single. Two of 5 qPCR optimistic circumstances had been smear unfavorable. Smear and T. evansi qPCR optimistic circumstances corresponded to animals that died regardless of being handled, indicating the affiliation of parasitemia with illness severity. This laboratory instrument will increase the potential of confirming outbreaks of kinetoplastid illnesses with zoonotic potential and determine the etiological brokers concerned.
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