To enrich RT-qPCR testing for analysis of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, many international locations have launched using fast antigen exams. As they typically show decrease real-life performances than anticipated, their right positioning as frontline screening continues to be controversial. Regardless of the shortage of information from day by day medical use, third era microfluidic assays (such because the LumiraDx SARS-CoV-2 Ag take a look at) have just lately been instructed to have comparable performances to RT-qPCR and have been proposed as various diagnostic instruments.
By analyzing 960 nasopharyngeal swabs from 960 topics on the emergency division admissions of a tertiary COVID-19 hospital, LumiraDx assay demonstrated a specificity of 97% (95% CI: 96-98), and a sensitivity of 85% (95% CI: 82-89) as compared with RT-qPCR, which will increase to 91% (95% CI: 86-95) for samples with a cycle threshold ≤ 29. Fifty false-negative LumiraDx-results have been confirmed by direct quantification of genomic SARS-CoV-2 RNA by way of droplet-digital PCR (median (IQR) load = 5880 (1657-41,440) copies/mL). Subgenomic N and E RNAs have been detected in 52% (n = 26) and 56% (n = 28) of them, respectively, supporting the presence of lively viral replication. Total, the LumiraDx take a look at complies with the minimal efficiency necessities of the WHO. But, the danger of a misrecognition of sufferers with lively COVID-19 persists, and the necessity for confirmatory RT-qPCR shouldn’t be amended.
Growth of a Tetraplex qPCR for the Molecular Identification and Quantification of Human Enteric Viruses, NoV and HAV, in Fish Samples
Human enteric viruses resembling norovirus (NoV) and hepatitis A virus (HAV) are a number of the most necessary causes of foodborne infections worldwide. Normally, an infection by way of fish consumption isn’t a priority concerning these viruses, since fish are primarily consumed cooked. Nevertheless, within the final years, uncooked fish consumption has turn into more and more widespread, particularly involving using seabass and gilthead seabream in dishes like sushi, sashimi, poke, and carpaccio.
Due to this fact, the danger for viral an infection by way of the consumption of uncooked fish has additionally elevated. On this research, a virologic screening was carried out in 323 fish specimens captured alongside the Portuguese coast utilizing a tetraplex qPCR optimised for 2 templates (plasmid and in vitro transcribed RNA) to detect and quantify NoV GI, NoV GII and HAV genomes. A distinction of roughly 1-log was discovered between using plasmid or in vitro transcribed RNA for molecular-based quantifications, exhibiting an underestimation of genome copy-number equivalents utilizing plasmid standard-based curves.
Moreover, the presence of NoV genomic RNA in a pool of seabass brains was recognized, which was proven to cluster with a serious group of human norovirus sequences from genogroup I (GI.1) by phylogenetic evaluation. Not one of the analysed fish revealed the presence of NoV GII or HAV. This end result corroborates the speculation that enteric viruses flow into in seawater or that fish have been contaminated throughout their transportation/dealing with, representing a possible threat to people by way of uncooked or undercooked fish consumption.
Screening of Reference Genes for RT-qPCR in Rooster Adipose Tissue and Adipocytes
Reverse transcription quantitative real-time PCR is essentially the most generally used technique to detect gene expression ranges. In experiments, it’s typically essential to right and standardize the expression degree of goal genes with reference genes. Due to this fact, it is rather necessary to pick secure reference genes to acquire correct quantitative outcomes. Though software examples of reference genes in mammals have been reported, no research have investigated using reference genes in finding out the expansion and growth of adipose tissue and the proliferation and differentiation of preadipocytes in chickens.
On this research, GeNorm, a reference gene stability statistical algorithm, was used to research the expression stability of 14 candidate reference genes within the stomach adipose tissue of broilers at 1, 4, and seven weeks of age, the proliferation and differentiation of main preadipocytes, in addition to instantly remoted preadipocytes and mature adipocytes. The outcomes confirmed that the expression of the TATA field binding protein (TBP) and hydroxymethylbilane synthase (HMBS) genes was most secure through the development and growth of stomach adipose tissue of broilers, the expression of the peptidylprolyl isomerase.
A (PPIA) and HMBS genes was most secure through the proliferation of main preadipocytes, the expression of the TBP and RPL13 genes was most secure through the differentiation of main preadipocytes, and the expression of the TBP and HMBS genes was most secure in instantly remoted preadipocytes and mature adipocytes. These outcomes present reference bases for precisely detecting the mRNA expression of useful genes in adipose tissue and adipocytes of chickens.
Stopping Chagas illness: A brand new RT-qPCR technique for fast and particular quantification of viable Trypanosoma cruzi for meals security
With out standardized strategies for quickly detecting in meals matrices viable T. cruzi, foodborne outbreaks stay uncared for. On this work, a reverse-transcriptase real-time PCR (RT-qPCR) mRNA-based approach was developed for the fast and particular detection and quantification of viable Trypanosoma cruzi in açai fruits and juice. The tactic makes use of particular primer concentrating on area on the cyt b gene.
 Green qPCR SuperMix |
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20-abx098031 |
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 Probe qPCR SuperMix |
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 Eva QPCR SuperMix Kit |
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K5052200 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Eva QPCR SuperMix Kit |
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K5052400 |
Biochain |
400 reactions |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 Power Eva QPCR SuperMix Kit |
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K5057200 |
Biochain |
200 reactions |
EUR 188 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Power Eva QPCR SuperMix Kit |
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K5057400 |
Biochain |
400 reactions |
EUR 320 |
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 Green qPCR SuperMix UDG |
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 Top Green qPCR SuperMix |
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 Tip Green qPCR SuperMix |
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 Library Quantification qPCR SuperMix |
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 cDNA Synthesis SuperMix for qPCR |
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) cDNA Synthesis SuperMix for qPCR (GC-rich) |
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EUR 718 |
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 HiScript II Q RT SuperMix for qPCR |
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R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
 HiScript II Q Select RT SuperMix for qPCR |
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R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 235 |
 PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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HS-100-QPCR |
CORNING |
100/pk |
EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
 PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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HS-150-QPCR |
CORNING |
100/pk |
EUR 318 |
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Description: Sealing Products; Sealing films - Axygen |
) HiScript III RT SuperMix for qPCR (+g DNA wiper) |
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R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 293 |
 QCell-Pro One-Step qRT-PCR SuperMix Kit |
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K5055200 |
Biochain |
200 reactions |
EUR 376 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
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K5055400 |
Biochain |
400 reactions |
EUR 572 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
) HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
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R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 244 |
 PCR SuperMix |
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20-abx098887 |
Abbexa |
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) T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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JT96-K002M |
T-Pro Biotechnology |
250ml*2/Kit |
EUR 204 |
T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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JT96-K002S |
T-Pro Biotechnology |
100ml*2/Kit |
EUR 152 |
) T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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JT96-K004M |
T-Pro Biotechnology |
250ml*2/Kit |
EUR 239 |
T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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JT96-K004S |
T-Pro Biotechnology |
100ml*2/Kit |
EUR 170 |
) T-Pro Endotoxin Removal Plasmid Midi kit (25) |
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RB94-EPI020 |
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20preps/Kit |
EUR 248 |
) T-Pro Endotoxin Removal Plasmid Maxi kit (10) |
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RB94-EPM010 |
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10preps/Kit |
EUR 265 |
) T-Pro Gel/PCR DNA Purification Maxi Kit (10) |
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10preps/Kit |
EUR 161 |
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RB94-NES100 |
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100preps/Kit |
EUR 161 |
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RB94-NES250 |
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EUR 222 |
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EUR 411.00
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) PCR SuperMix (+dye) |
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20-abx098003 |
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EUR 411.00
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EUR 6174.00
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 cDNA Synthesis SuperMix |
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Abbexa |
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20-abx098888 |
Abbexa |
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 Library Amplification SuperMix |
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20-abx098894 |
Abbexa |
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 OneScriptcDNA Synthesis SuperMix |
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G451 |
ABM |
25 x 20 ul reactions |
EUR 89 |
OneScriptcDNA Synthesis SuperMix |
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G452 |
ABM |
100 x 20 ul reactions |
EUR 141 |
 Evo? cDNA Supermix |
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M1168-100 |
Biovision |
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EUR 381 |
Evo? cDNA Supermix |
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M1168-25 |
Biovision |
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EUR 267 |
 Novo? cDNA Supermix |
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M1169-100 |
Biovision |
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EUR 441 |
Novo? cDNA Supermix |
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M1169-25 |
Biovision |
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EUR 289 |
) T-Pro Nonliposomal Transfection Reagent I (NTR I) |
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JT97-N001M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 187 |
) T-Pro Nonliposomal Transfection Reagent II (NTR II) |
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JT97-N002M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 204 |
) T-Pro Nonliposomal Transfection Reagent III (NTR III) |
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JT97-N006M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 222 |
 Entrans qPCR Probe Kit |
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RK21209 |
Abclonal |
40 RXN |
Ask for price |
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 qPCR Control Template Kit |
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G929 |
ABM |
100 rxns |
EUR 148 |
 Lentivirus qPCR Quantification Kit |
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K1471-100 |
Biovision |
100 Rxns |
EUR 298 |
 Retrovirus qPCR Quantification Kit |
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K1472-100 |
Biovision |
100 Rxns |
EUR 318 |
 QPCR Positive Control Kit |
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M1126-100 |
Biovision |
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EUR 403 |
 Reagent (non-reducing 4X)) T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X) |
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JB06-F003 |
T-Pro Biotechnology |
10ml/BT |
EUR 126 |
) T PCR SuperMix (-dye) |
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20-abx098005 |
Abbexa |
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) T PCR SuperMix (+dye) |
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20-abx098006 |
Abbexa |
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) EasyPfu PCR SuperMix (-dye) |
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20-abx098009 |
Abbexa |
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) FastPfu PCR SuperMix (-dye) |
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20-abx098010 |
Abbexa |
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 OneScriptPlus cDNA Synthesis SuperMix |
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G453 |
ABM |
25 x 20 ul reactions |
EUR 97 |
OneScriptPlus cDNA Synthesis SuperMix |
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G454 |
ABM |
100 x 20 ul reactions |
EUR 169 |
 AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS |
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AP-STR-KIT-P |
CORNING |
1/pk |
EUR 721 |
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Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
 for western blot washing) T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing |
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JB09-I001 |
T-Pro Biotechnology |
500ml/BT |
EUR 135 |
 for western blot washing) T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing |
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JB09-I002 |
T-Pro Biotechnology |
500ml/BT |
EUR 135 |
 Methylamp MS-qPCR Fast Kit |
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P-1028 |
EpiGentek |
200 Reactions |
EUR 496.6 |
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Description: fast delivery possible |
 Universal qPCR Mixture |
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QPCR1000-UNIV |
Bio Basic |
4X1.25ml |
EUR 276.2 |
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 Frit Kit |
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FRIT-KIT |
Next Advance |
1each |
EUR 124 |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
 20 profiles) LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
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RA900A-1 |
SBI |
20 profiles |
EUR 850 |
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 Fmoc-Pro-Pro-Pro-Pro-Pro-OH |
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B-3660.0050 |
Bachem |
50.0mg |
EUR 176 |
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Description: Sum Formula: C40H47N5O8; CAS# [454693-94-8] |
Fmoc-Pro-Pro-Pro-Pro-Pro-OH |
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B-3660.0250 |
Bachem |
250.0mg |
EUR 611 |
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Description: Sum Formula: C40H47N5O8; CAS# [454693-94-8] |
) PCR SuperMix for PAGE (+dye) |
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20-abx098004 |
Abbexa |
-
EUR 411.00
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EUR 189.00
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EUR 258.00
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 Column Packing Kit |
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PACK-KIT |
Next Advance |
1pack |
EUR 1035 |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
 PCR Mycoplasma Detection Kit |
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M034-Kit |
TOKU-E |
Kit |
EUR 266 |
 Boc-Pro-Pro-Pro-Pro-OH |
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A-2615.0100 |
Bachem |
100.0mg |
EUR 212 |
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Description: Sum Formula: C25H38N4O7; CAS# [29804-52-2] |
Boc-Pro-Pro-Pro-Pro-OH |
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A-2615.0250 |
Bachem |
250.0mg |
EUR 393 |
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Description: Sum Formula: C25H38N4O7; CAS# [29804-52-2] |
 Fmoc-Pro-Pro-Pro-Pro-OH |
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B-3680.0050 |
Bachem |
50.0mg |
EUR 162 |
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Description: Sum Formula: C35H40N4O7; CAS# [454693-96-0] |
Fmoc-Pro-Pro-Pro-Pro-OH |
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B-3680.0250 |
Bachem |
250.0mg |
EUR 563 |
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Description: Sum Formula: C35H40N4O7; CAS# [454693-96-0] |
 H-Pro-Pro-Pro-Pro-OH |
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H-4800.0050 |
Bachem |
50.0mg |
EUR 297 |
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Description: Sum Formula: C20H30N4O5; CAS# [21866-90-0] |
H-Pro-Pro-Pro-Pro-OH |
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H-4800.0250 |
Bachem |
250.0mg |
EUR 1093 |
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Description: Sum Formula: C20H30N4O5; CAS# [21866-90-0] |
 20 profiles) Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
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RA930A-1 |
SBI |
20 profiles |
EUR 850 |
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 Coronavirus Rapid RT-qPCR Detection Kit |
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K1461-100 |
Biovision |
100 Rxns |
EUR 551 |
 Adeno-Associated Virus qPCR Quantification Kit |
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K1473-100 |
Biovision |
100 Rxns |
EUR 348 |
 Denosumab (Prolia) Qualitative ELISA kit) Shikari (S-ATD) Denosumab (Prolia) Qualitative ELISA kit |
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DEN-QLS-PRO |
Matriks Biotek |
1x96-wells test plate |
EUR 832.5 |
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Description: Enzyme Immunoassay for Qualitative detection of Denosumab (Prolia) in human serum and plasma samples. |
 Denosumab (Prolia)Free drug ELISA kit) Shikari (Q-DEN) Denosumab (Prolia)Free drug ELISA kit |
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DEN-FD-PRO |
Matriks Biotek |
1x96-wells test plate |
EUR 878 |
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Description: Enzyme Immunoassay for detection of freeDenosumab (Prolia) in human serum and plasma samples. |
 QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054200 |
Biochain |
200 reactions |
EUR 408 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054400 |
Biochain |
400 reactions |
EUR 594 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 Entrans qPCR Probe Set |
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RK21210 |
Abclonal |
40 RXN |
Ask for price |
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 Neural Lineage qPCR Profiler |
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RA500A-1 |
SBI |
10 reactions |
EUR 999 |
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 Cancer MicroRNA qPCR Array |
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RA610A-1 |
SBI |
10 profiles |
EUR 1172 |
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 Regulatory RNA qPCR Profiler |
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RA950A-1 |
SBI |
20 rxn |
EUR 880 |
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 Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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CAS400A-KIT |
SBI |
1 kit (10 rxn) |
EUR 1110 |
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 Fmoc-Pro-Pro-Pro-OH |
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B-3690.0050 |
Bachem |
50.0mg |
EUR 151 |
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Description: Sum Formula: C30H33N3O6; CAS# [134303-96-1] |
Fmoc-Pro-Pro-Pro-OH |
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B-3690.0250 |
Bachem |
250.0mg |
EUR 515 |
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Description: Sum Formula: C30H33N3O6; CAS# [134303-96-1] |
 H-Pro-Pro-Pro-OH |
|
H-4795.0250 |
Bachem |
250.0mg |
EUR 683 |
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Description: Sum Formula: C15H23N3O4; CAS# [19285-44-0] |
H-Pro-Pro-Pro-OH |
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H-4795.1000 |
Bachem |
1.0g |
EUR 1989 |
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Description: Sum Formula: C15H23N3O4; CAS# [19285-44-0] |
 H-Pro-Pro-Pro-Gly-Met-Arg-Pro-Pro-OH |
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H-3464.0001 |
Bachem |
1.0mg |
EUR 303 |
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Description: Sum Formula: C38H61N11O9S; CAS# [141312-42-7] net |
H-Pro-Pro-Pro-Gly-Met-Arg-Pro-Pro-OH |
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H-3464.0005 |
Bachem |
5.0mg |
EUR 1118 |
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Description: Sum Formula: C38H61N11O9S; CAS# [141312-42-7] net |
 miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-20rxns20ulSystems |
Abbexa |
20 rxns × 20 ul Systems |
EUR 732 |
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 Green Two-Step qRT-PCR SuperMix |
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abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 648 |
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 Green One-Step qRT-PCR SuperMix |
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20-abx09803820ulSystems |
Abbexa |
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- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
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 Probe One-Step qRT-PCR SuperMix |
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20-abx09804220ulSystems |
Abbexa |
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- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
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 Fly First-Strand cDNA Synthesis SuperMix |
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20-abx09885820ulSystems |
Abbexa |
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- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
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 All-in-One cDNA Synthesis SuperMix |
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B24403 |
Bimake |
200 ractions |
EUR 456 |
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Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
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B24408 |
Bimake |
1000 ractions |
EUR 1453 |
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Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
 CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV100PA-KIT |
SBI |
1 Kit |
EUR 1132 |
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 CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV105PA-KIT |
SBI |
1 Kit |
EUR 1132 |
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 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV120PA-KIT |
SBI |
1 Kit |
EUR 1132 |
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 MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV125PA-KIT |
SBI |
1 Kit |
EUR 1132 |
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 Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS700A-KIT |
SBI |
10 rxn |
EUR 1132 |
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 Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS720A-KIT |
SBI |
10 rxn |
EUR 1132 |
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 Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS740A-KIT |
SBI |
10 rxn |
EUR 1132 |
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The utmost restoration price of T. cruzi from inoculated açai juice was 82.50%. The restrict of detection and quantification in açai juice was 10 parasites/mL for RT-qPCR (mRNA-based) and qPCR (DNA-based). The RT-qPCR effectivity was estimated at 97.27% with an R2 of 0.994. The RT-qPCR was proven to have the ability to discriminate between viable and nonviable cells. This technique supplies a great tool for fast evaluation of low concentrations of viable T. cruzi in naturally contaminated meals samples, and may be utilized industrially as a high quality and safety technique.
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