To enrich RT-qPCR testing for analysis of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, many international locations have launched using fast antigen exams. As they typically show decrease real-life performances than anticipated, their right positioning as frontline screening continues to be controversial. Regardless of the shortage of information from day by day medical use, third era microfluidic assays (such because the LumiraDx SARS-CoV-2 Ag take a look at) have just lately been instructed to have comparable performances to RT-qPCR and have been proposed as various diagnostic instruments.
By analyzing 960 nasopharyngeal swabs from 960 topics on the emergency division admissions of a tertiary COVID-19 hospital, LumiraDx assay demonstrated a specificity of 97% (95% CI: 96-98), and a sensitivity of 85% (95% CI: 82-89) as compared with RT-qPCR, which will increase to 91% (95% CI: 86-95) for samples with a cycle threshold ≤ 29. Fifty false-negative LumiraDx-results have been confirmed by direct quantification of genomic SARS-CoV-2 RNA by way of droplet-digital PCR (median (IQR) load = 5880 (1657-41,440) copies/mL). Subgenomic N and E RNAs have been detected in 52% (n = 26) and 56% (n = 28) of them, respectively, supporting the presence of lively viral replication. Total, the LumiraDx take a look at complies with the minimal efficiency necessities of the WHO. But, the danger of a misrecognition of sufferers with lively COVID-19 persists, and the necessity for confirmatory RT-qPCR shouldn’t be amended.

Growth of a Tetraplex qPCR for the Molecular Identification and Quantification of Human Enteric Viruses, NoV and HAV, in Fish Samples

Human enteric viruses resembling norovirus (NoV) and hepatitis A virus (HAV) are a number of the most necessary causes of foodborne infections worldwide. Normally, an infection by way of fish consumption isn’t a priority concerning these viruses, since fish are primarily consumed cooked. Nevertheless, within the final years, uncooked fish consumption has turn into more and more widespread, particularly involving using seabass and gilthead seabream in dishes like sushi, sashimi, poke, and carpaccio.
Due to this fact, the danger for viral an infection by way of the consumption of uncooked fish has additionally elevated. On this research, a virologic screening was carried out in 323 fish specimens captured alongside the Portuguese coast utilizing a tetraplex qPCR optimised for 2 templates (plasmid and in vitro transcribed RNA) to detect and quantify NoV GI, NoV GII and HAV genomes. A distinction of roughly 1-log was discovered between using plasmid or in vitro transcribed RNA for molecular-based quantifications, exhibiting an underestimation of genome copy-number equivalents utilizing plasmid standard-based curves.
 Frontline Screening for SARS-CoV-2 Infection at Emergency Department Admission by Third Generation Rapid Antigen Test: Can We Spare RT-qPCR?
Moreover, the presence of NoV genomic RNA in a pool of seabass brains was recognized, which was proven to cluster with a serious group of human norovirus sequences from genogroup I (GI.1) by phylogenetic evaluation. Not one of the analysed fish revealed the presence of NoV GII or HAV. This end result corroborates the speculation that enteric viruses flow into in seawater or that fish have been contaminated throughout their transportation/dealing with, representing a possible threat to people by way of uncooked or undercooked fish consumption.

Screening of Reference Genes for RT-qPCR in Rooster Adipose Tissue and Adipocytes

Reverse transcription quantitative real-time PCR is essentially the most generally used technique to detect gene expression ranges. In experiments, it’s typically essential to right and standardize the expression degree of goal genes with reference genes. Due to this fact, it is rather necessary to pick secure reference genes to acquire correct quantitative outcomes. Though software examples of reference genes in mammals have been reported, no research have investigated using reference genes in finding out the expansion and growth of adipose tissue and the proliferation and differentiation of preadipocytes in chickens.
On this research, GeNorm, a reference gene stability statistical algorithm, was used to research the expression stability of 14 candidate reference genes within the stomach adipose tissue of broilers at 1, 4, and seven weeks of age, the proliferation and differentiation of main preadipocytes, in addition to instantly remoted preadipocytes and mature adipocytes. The outcomes confirmed that the expression of the TATA field binding protein (TBP) and hydroxymethylbilane synthase (HMBS) genes was most secure through the development and growth of stomach adipose tissue of broilers, the expression of the peptidylprolyl isomerase.
A (PPIA) and HMBS genes was most secure through the proliferation of main preadipocytes, the expression of the TBP and RPL13 genes was most secure through the differentiation of main preadipocytes, and the expression of the TBP and HMBS genes was most secure in instantly remoted preadipocytes and mature adipocytes. These outcomes present reference bases for precisely detecting the mRNA expression of useful genes in adipose tissue and adipocytes of chickens.

Stopping Chagas illness: A brand new RT-qPCR technique for fast and particular quantification of viable Trypanosoma cruzi for meals security

With out standardized strategies for quickly detecting in meals matrices viable T. cruzi, foodborne outbreaks stay uncared for. On this work, a reverse-transcriptase real-time PCR (RT-qPCR) mRNA-based approach was developed for the fast and particular detection and quantification of viable Trypanosoma cruzi in açai fruits and juice. The tactic makes use of particular primer concentrating on area on the cyt b gene.

Green qPCR SuperMix

20-abx098031
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
  • 5 ml

Probe qPCR SuperMix

20-abx098040
  • EUR 1038.00
  • EUR 439.00
  • EUR 634.00
  • 15 ml
  • 1 ml
  • 5 ml

Eva QPCR SuperMix Kit

K5052200 200 reactions
EUR 211
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Eva QPCR SuperMix Kit

K5052400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Power Eva QPCR SuperMix Kit

K5057200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Power Eva QPCR SuperMix Kit

K5057400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Green qPCR SuperMix UDG

20-abx098032
  • EUR 1135.00
  • EUR 453.00
  • EUR 662.00
  • 15 ml
  • 1 ml
  • 5 ml

Top Green qPCR SuperMix

20-abx098033
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix

20-abx098034
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Library Quantification qPCR SuperMix

20-abx098896
  • EUR 230.00
  • EUR 411.00
  • 1 ml
  • 5 ml

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

Top Green qPCR SuperMix (+Dye II)

20-abx098890
  • EUR 829.00
  • EUR 217.00
  • EUR 1135.00
  • EUR 411.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

Tip Green qPCR SuperMix (+Dye II)

20-abx098891
  • EUR 968.00
  • EUR 230.00
  • EUR 1330.00
  • EUR 467.00
  • 15 ml
  • 1 ml
  • 25 ml
  • 5 ml

HiScript II Q RT SuperMix for qPCR

R222-01 100 rxn (20 μl/rxn)
EUR 235

cDNA Synthesis SuperMix for qPCR (GC-rich)

abx098024-50rxns20ulSystems 50 rxns × 20 ul Systems
EUR 718

HiScript II Q Select RT SuperMix for qPCR

R232-01 100 rxn (20 μl/rxn)
EUR 235

PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500

HS-100-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

HiScript III RT SuperMix for qPCR (+g DNA wiper)

R323-01 100 rxn (20 μl/rxn)
EUR 293

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055200 200 reactions
EUR 376
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Pro One-Step qRT-PCR SuperMix Kit

K5055400 400 reactions
EUR 572
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper)

R233-01 100 rxn (20 μl/rxn)
EUR 244

PCR SuperMix

20-abx098887
  • EUR 272.00
  • EUR 578.00
  • 1 ml
  • 5 ml

T-Pro BCA Protein Assay kit

JB04-D001 500assay/KIT
EUR 204

T-Pro Plasmid Mini Kit (100)

RB94-YPD100 100preps/Kit
EUR 161

T-Pro Plasmid Mini Kit (250)

RB94-YPD250 250preps/Kit
EUR 222

T-Pro Plasmid Midi Kit (20)

RB94-YPI020 20preps/Kit
EUR 213

T-Pro Plasmid Maxi Kit (10)

RB94-YPM010 10preps/Kit
EUR 222

T-Pro Bradford Protein Assay kit(1X)

JB04-D002 500ml/BT
EUR 161

T-Pro Genomic DNA Midi Kit (20)

RB94-NGM020 20preps/kit
EUR 222

T-Pro Genomic DNA Mini Kit (100)

RB94-NGS100 100preps/kit
EUR 213

T-Pro Stacking Buffer

JB03-C002 500ml/BT
EUR 152

T-Pro MycoClean spray

JT90-R002 500ml/BT
EUR 144

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K002M 250ml*2/Kit
EUR 204

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K002S 100ml*2/Kit
EUR 152

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K004M 250ml*2/Kit
EUR 239

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K004S 100ml*2/Kit
EUR 170

OneScriptcDNA Synthesis SuperMix

G451 25 x 20 ul reactions
EUR 89

OneScriptcDNA Synthesis SuperMix

G452 100 x 20 ul reactions
EUR 141

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

PCR SuperMix (-dye)

20-abx098002
  • EUR 411.00
  • EUR 189.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 5 ml

PCR SuperMix (+dye)

20-abx098003
  • EUR 411.00
  • EUR 189.00
  • EUR 6174.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 480 ml
  • 5 ml

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Plus PCR SuperMix

20-abx098888
  • EUR 244.00
  • EUR 467.00
  • 1 ml
  • 5 ml

Library Amplification SuperMix

20-abx098894
  • EUR 258.00
  • EUR 537.00
  • 1 ml
  • 5 ml

T-Pro Endotoxin Removal Plasmid Midi kit (25)

RB94-EPI020 20preps/Kit
EUR 248

T-Pro Endotoxin Removal Plasmid Maxi kit (10)

RB94-EPM010 10preps/Kit
EUR 265

T-Pro Plant Genomic DNA Midi Kit (20)

RB94-PGM020 20preps/kit
EUR 222

T-Pro Plant Genomic DNA Mini Kit (100)

RB94-PGS100 100preps/kit
EUR 222

T-Pro Gel/PCR DNA Purification Maxi Kit (10)

RB94-NEL010 10preps/Kit
EUR 161

T-Pro Gel/PCR DNA Purification Mini Kit (100)

RB94-NES100 100preps/Kit
EUR 161

T-Pro Gel/PCR DNA Purification Mini Kit (250)

RB94-NES250 250preps/Kit
EUR 222

T-Pro EZ Gel Solution 8%

JB02-B008M 500ml/BT
EUR 222

T-Pro EZ Gel Solution 8%

JB02-B008S 100ml/BT
EUR 135

T-Pro EZ Gel Solution 10%

JB02-B010M 500ml/BT
EUR 222

T-Pro EZ Gel Solution 10%

JB02-B010S 100ml/BT
EUR 135

T-Pro EZ Gel Solution 12%

JB02-B012M 500ml/BT
EUR 222

T-Pro EZ Gel Solution 12%

JB02-B012S 100ml/BT
EUR 135

T-Pro EZ Gel Solution 15%

JB02-B015M 500ml/BT
EUR 222

T-Pro EZ Gel Solution 15%

JB02-B015S 100ml/BT
EUR 135

T-Pro Separating or Resolving Buffer

JB03-C001 500ml/BT
EUR 152

T-Pro Transfer Blotting buffer (10X)

JB08-H001 500ml/BT
EUR 135

T-Pro Western Blot Stripping Reagent

JB11-K002 500ml/BT
EUR 178

T-Pro Aqua EZ Clean (M)

JT90-R001M 500ml/BT
EUR 332

T-Pro Aqua EZ Clean (S)

JT90-R001S 100ml/BT
EUR 151

T-Pro EZ stain Gel solution

JT90-R005M 500ml/BT
EUR 187

T-Pro EZ stain Gel solution

JT90-R005S 100ml/BT
EUR 126

T-Pro P-Fect Transfection Reagent

JT97-N005M 1.0ml/vial
EUR 222

qPCR Control Template Kit

G929 100 rxns
EUR 148

Lentivirus qPCR Quantification Kit

K1471-100 100 Rxns
EUR 298

Retrovirus qPCR Quantification Kit

K1472-100 100 Rxns Ask for price

QPCR Positive Control Kit

M1126-100
EUR 403

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

T PCR SuperMix (-dye)

20-abx098005
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml

T PCR SuperMix (+dye)

20-abx098006
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml

EasyPfu PCR SuperMix (-dye)

20-abx098009
  • EUR 203.00
  • EUR 342.00
  • 1 ml
  • 5 ml

FastPfu PCR SuperMix (-dye)

20-abx098010
  • EUR 244.00
  • EUR 495.00
  • 1 ml
  • 5 ml

T-Pro BCA Protein Assay Reagent A

JB04-D001A 500ml/BT
EUR 170

T-Pro BCA Protein Assay Reagent B

JB04-D001B 12ml/BT
EUR 117

T-Pro Fast Blocking Buffer (in PBS)

JK92-W001 500ml/BT
EUR 187

T-Pro Fast Blocking Buffer (in TBS)

JK92-W002 500ml/BT
EUR 187

T-Pro Laemmli (SDS sample) Reagent (reducing 4X)

JB06-F002 10ml/BT
EUR 126

T-Pro Tris-Glycine-SDS running buffer (10X)

JB07-G001 500ml/BT
EUR 135

T-Pro Tris-Glycine-Native running buffer (10X)

JB07-G002 500ml/BT
EUR 135

T-Pro Semi Dry Transfer Blotting buffer (10X)

JB08-H002 500ml/BT
EUR 144

T-Pro Protein Free Blocking Buffer (in PBS)

JK92-W003 500ml/BT
EUR 187

T-Pro Protein Free Blocking Buffer (in TBS)

JK92-W004 500ml/BT
EUR 187

T-Pro Nonliposomal Transfection Reagent I (NTR I)

JT97-N001M 1.0ml/vial
EUR 187

T-Pro Nonliposomal Transfection Reagent II (NTR II)

JT97-N002M 1.0ml/vial
EUR 204

T-Pro Nonliposomal Transfection Reagent III (NTR III)

JT97-N006M 1.0ml/vial
EUR 222

Methylamp MS-qPCR Fast Kit

P-1028 200 Reactions
EUR 496.6
Description: fast delivery possible

AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS

AP-STR-KIT-P 1/pk
EUR 721
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X)

JB06-F003 10ml/BT
EUR 126

Universal qPCR Mixture

QPCR1000-UNIV 4X1.25ml
EUR 276.2

PCR SuperMix for PAGE (+dye)

20-abx098004
  • EUR 411.00
  • EUR 189.00
  • EUR 258.00
  • 15 ml
  • 1 ml
  • 5 ml

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

T-Pro Washing buffer in PBS and Tween-20 (10X)

JB09-I003 500ml/BT
EUR 144

T-Pro Washing buffer in TBS and Tween-20 (10X)

JB09-I004 500ml/BT
EUR 144

LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA900A-1 20 profiles
EUR 850

Shikari (S-ATD) Denosumab (Prolia) Qualitative ELISA kit

DEN-QLS-PRO 1x96-wells test plate
EUR 832.5
Description: Enzyme Immunoassay for Qualitative detection of Denosumab (Prolia) in human serum and plasma samples.

Shikari (Q-DEN) Denosumab (Prolia)Free drug ELISA kit

DEN-FD-PRO 1x96-wells test plate
EUR 878
Description: Enzyme Immunoassay for detection of freeDenosumab (Prolia) in human serum and plasma samples.

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing

JB09-I001 500ml/BT
EUR 135

T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing

JB09-I002 500ml/BT
EUR 135

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles

RA930A-1 20 profiles
EUR 850

Coronavirus Rapid RT-qPCR Detection Kit

K1461-100 100 Rxns
EUR 551

Adeno-Associated Virus qPCR Quantification Kit

K1473-100 100 Rxns
EUR 348

igScript RT-qPCR Kit - 50 Reactions

4513 1/EA
EUR 248

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054200 200 reactions
EUR 408
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QCell-Eva One-Step qRT-PCR SuperMix Kit

K5054400 400 reactions
EUR 594
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Neural Lineage qPCR Profiler

RA500A-1 10 reactions
EUR 999

Cancer MicroRNA qPCR Array

RA610A-1 10 profiles
EUR 1172

Regulatory RNA qPCR Profiler

RA950A-1 20 rxn
EUR 880

All-in-One cDNA Synthesis SuperMix

B24403 200 ractions
EUR 456
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix

B24408 1000 ractions
EUR 1453
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

miRNA First-Strand cDNA Synthesis SuperMix

abx098020-20rxns20ulSystems 20 rxns × 20 ul Systems
EUR 732

Green Two-Step qRT-PCR SuperMix

abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)
EUR 648

Green One-Step qRT-PCR SuperMix

20-abx09803820ulSystems
  • EUR 495.00
  • EUR 746.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Probe One-Step qRT-PCR SuperMix

20-abx09804220ulSystems
  • EUR 592.00
  • EUR 1024.00
  • 100 rxns × 20 ul Systems
  • 400 rxns × 20 ul Systems

Fly First-Strand cDNA Synthesis SuperMix

20-abx09885820ulSystems
  • EUR 718.00
  • EUR 578.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110

Fmoc-Pro-Pro-Pro-Pro-Pro-OH

B-3660.0050 50.0mg
EUR 176
Description: Sum Formula: C40H47N5O8; CAS# [454693-94-8]

Fmoc-Pro-Pro-Pro-Pro-Pro-OH

B-3660.0250 250.0mg
EUR 611
Description: Sum Formula: C40H47N5O8; CAS# [454693-94-8]

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805

Boc-Pro-Pro-Pro-Pro-OH

A-2615.0100 100.0mg
EUR 212
Description: Sum Formula: C25H38N4O7; CAS# [29804-52-2]

Boc-Pro-Pro-Pro-Pro-OH

A-2615.0250 250.0mg
EUR 393
Description: Sum Formula: C25H38N4O7; CAS# [29804-52-2]

Fmoc-Pro-Pro-Pro-Pro-OH

B-3680.0050 50.0mg
EUR 162
Description: Sum Formula: C35H40N4O7; CAS# [454693-96-0]

Fmoc-Pro-Pro-Pro-Pro-OH

B-3680.0250 250.0mg
EUR 563
Description: Sum Formula: C35H40N4O7; CAS# [454693-96-0]

H-Pro-Pro-Pro-Pro-OH

H-4800.0050 50.0mg
EUR 297
Description: Sum Formula: C20H30N4O5; CAS# [21866-90-0]

H-Pro-Pro-Pro-Pro-OH

H-4800.0250 250.0mg
EUR 1093
Description: Sum Formula: C20H30N4O5; CAS# [21866-90-0]

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1132

One.Step RT-qPCR Probe Kit (2X concentrated)

105-500 2 x 1,25 ml
EUR 458

One.Step RT-qPCR Probe Kit (2X concentrated)

105-502 10 x 1,25 ml
EUR 1719
The utmost restoration price of T. cruzi from inoculated açai juice was 82.50%. The restrict of detection and quantification in açai juice was 10 parasites/mL for RT-qPCR (mRNA-based) and qPCR (DNA-based). The RT-qPCR effectivity was estimated at 97.27% with an R2 of 0.994. The RT-qPCR was proven to have the ability to discriminate between viable and nonviable cells. This technique supplies a great tool for fast evaluation of low concentrations of viable T. cruzi in naturally contaminated meals samples, and may be utilized industrially as a high quality and safety technique.