To enrich RT-qPCR testing for analysis of extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, many international locations have launched using fast antigen exams. As they typically show decrease real-life performances than anticipated, their right positioning as frontline screening continues to be controversial. Regardless of the shortage of information from day by day medical use, third era microfluidic assays (such because the LumiraDx SARS-CoV-2 Ag take a look at) have just lately been instructed to have comparable performances to RT-qPCR and have been proposed as various diagnostic instruments.
By analyzing 960 nasopharyngeal swabs from 960 topics on the emergency division admissions of a tertiary COVID-19 hospital, LumiraDx assay demonstrated a specificity of 97% (95% CI: 96-98), and a sensitivity of 85% (95% CI: 82-89) as compared with RT-qPCR, which will increase to 91% (95% CI: 86-95) for samples with a cycle threshold ≤ 29. Fifty false-negative LumiraDx-results have been confirmed by direct quantification of genomic SARS-CoV-2 RNA by way of droplet-digital PCR (median (IQR) load = 5880 (1657-41,440) copies/mL). Subgenomic N and E RNAs have been detected in 52% (n = 26) and 56% (n = 28) of them, respectively, supporting the presence of lively viral replication. Total, the LumiraDx take a look at complies with the minimal efficiency necessities of the WHO. But, the danger of a misrecognition of sufferers with lively COVID-19 persists, and the necessity for confirmatory RT-qPCR shouldn’t be amended.
Growth of a Tetraplex qPCR for the Molecular Identification and Quantification of Human Enteric Viruses, NoV and HAV, in Fish Samples
Human enteric viruses resembling norovirus (NoV) and hepatitis A virus (HAV) are a number of the most necessary causes of foodborne infections worldwide. Normally, an infection by way of fish consumption isn’t a priority concerning these viruses, since fish are primarily consumed cooked. Nevertheless, within the final years, uncooked fish consumption has turn into more and more widespread, particularly involving using seabass and gilthead seabream in dishes like sushi, sashimi, poke, and carpaccio.
Due to this fact, the danger for viral an infection by way of the consumption of uncooked fish has additionally elevated. On this research, a virologic screening was carried out in 323 fish specimens captured alongside the Portuguese coast utilizing a tetraplex qPCR optimised for 2 templates (plasmid and in vitro transcribed RNA) to detect and quantify NoV GI, NoV GII and HAV genomes. A distinction of roughly 1-log was discovered between using plasmid or in vitro transcribed RNA for molecular-based quantifications, exhibiting an underestimation of genome copy-number equivalents utilizing plasmid standard-based curves.
Moreover, the presence of NoV genomic RNA in a pool of seabass brains was recognized, which was proven to cluster with a serious group of human norovirus sequences from genogroup I (GI.1) by phylogenetic evaluation. Not one of the analysed fish revealed the presence of NoV GII or HAV. This end result corroborates the speculation that enteric viruses flow into in seawater or that fish have been contaminated throughout their transportation/dealing with, representing a possible threat to people by way of uncooked or undercooked fish consumption.
Screening of Reference Genes for RT-qPCR in Rooster Adipose Tissue and Adipocytes
Reverse transcription quantitative real-time PCR is essentially the most generally used technique to detect gene expression ranges. In experiments, it’s typically essential to right and standardize the expression degree of goal genes with reference genes. Due to this fact, it is rather necessary to pick secure reference genes to acquire correct quantitative outcomes. Though software examples of reference genes in mammals have been reported, no research have investigated using reference genes in finding out the expansion and growth of adipose tissue and the proliferation and differentiation of preadipocytes in chickens.
On this research, GeNorm, a reference gene stability statistical algorithm, was used to research the expression stability of 14 candidate reference genes within the stomach adipose tissue of broilers at 1, 4, and seven weeks of age, the proliferation and differentiation of main preadipocytes, in addition to instantly remoted preadipocytes and mature adipocytes. The outcomes confirmed that the expression of the TATA field binding protein (TBP) and hydroxymethylbilane synthase (HMBS) genes was most secure through the development and growth of stomach adipose tissue of broilers, the expression of the peptidylprolyl isomerase.
A (PPIA) and HMBS genes was most secure through the proliferation of main preadipocytes, the expression of the TBP and RPL13 genes was most secure through the differentiation of main preadipocytes, and the expression of the TBP and HMBS genes was most secure in instantly remoted preadipocytes and mature adipocytes. These outcomes present reference bases for precisely detecting the mRNA expression of useful genes in adipose tissue and adipocytes of chickens.
Stopping Chagas illness: A brand new RT-qPCR technique for fast and particular quantification of viable Trypanosoma cruzi for meals security
With out standardized strategies for quickly detecting in meals matrices viable T. cruzi, foodborne outbreaks stay uncared for. On this work, a reverse-transcriptase real-time PCR (RT-qPCR) mRNA-based approach was developed for the fast and particular detection and quantification of viable Trypanosoma cruzi in açai fruits and juice. The tactic makes use of particular primer concentrating on area on the cyt b gene.
Eva QPCR SuperMix Kit |
K5052200 |
Biochain |
200 reactions |
EUR 211 |
Eva QPCR SuperMix Kit |
K5052400 |
Biochain |
400 reactions |
EUR 354 |
Probe qPCR SuperMix |
20-abx098040 |
Abbexa |
-
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|
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Fast Eva QPCR SuperMix Kit |
K5052002 |
Biochain |
200 rxn |
EUR 211 |
Power Eva QPCR SuperMix Kit |
K5057200 |
Biochain |
200 reactions |
EUR 180 |
Power Eva QPCR SuperMix Kit |
K5057400 |
Biochain |
400 reactions |
EUR 354 |
Green qPCR SuperMix |
20-abx098031 |
Abbexa |
-
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|
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Green qPCR SuperMix |
abx098031-100l |
Abbexa |
100 µl |
EUR 675 |
Green qPCR SuperMix |
abx098031-200l |
Abbexa |
200 µl |
EUR 912.5 |
Green qPCR SuperMix UDG |
20-abx098032 |
Abbexa |
-
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Top Green qPCR SuperMix |
20-abx098033 |
Abbexa |
-
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Tip Green qPCR SuperMix |
20-abx098034 |
Abbexa |
-
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Green qPCR SuperMix UDG |
abx098032-100l |
Abbexa |
100 µl |
EUR 687.5 |
Green qPCR SuperMix UDG |
abx098032-200l |
Abbexa |
200 µl |
EUR 912.5 |
Top Green qPCR SuperMix |
abx098033-100l |
Abbexa |
100 µl |
EUR 262.5 |
Top Green qPCR SuperMix |
abx098033-200l |
Abbexa |
200 µl |
EUR 487.5 |
Tip Green qPCR SuperMix |
abx098034-100l |
Abbexa |
100 µl |
EUR 262.5 |
Tip Green qPCR SuperMix |
abx098034-200l |
Abbexa |
200 µl |
EUR 437.5 |
EnTurbo™ probe qPCR SuperMix |
EQ017-25mL |
ELK Biotech |
25mL |
EUR 240 |
EnTurbo™ probe qPCR SuperMix |
EQ017-5mL |
ELK Biotech |
5mL |
EUR 78 |
EnTurbo™ probe qPCR SuperMix |
EQ017 |
ELK Biotech |
5mL |
EUR 240 |
Library Quantification qPCR SuperMix |
20-abx098896 |
Abbexa |
-
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Library Quantification qPCR SuperMix |
abx098896-100l |
Abbexa |
100 µl |
EUR 700 |
Library Quantification qPCR SuperMix |
abx098896-200l |
Abbexa |
200 µl |
EUR 962.5 |
Top Green qPCR SuperMix (+Dye II) |
20-abx098890 |
Abbexa |
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Tip Green qPCR SuperMix (+Dye II) |
20-abx098891 |
Abbexa |
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Top Green qPCR SuperMix (+Dye II) |
abx098890-100l |
Abbexa |
100 µl |
EUR 262.5 |
Top Green qPCR SuperMix (+Dye II) |
abx098890-200l |
Abbexa |
200 µl |
EUR 487.5 |
Tip Green qPCR SuperMix (+Dye II) |
abx098891-100l |
Abbexa |
100 µl |
EUR 262.5 |
Tip Green qPCR SuperMix (+Dye II) |
abx098891-200l |
Abbexa |
200 µl |
EUR 437.5 |
EnTurbo™ SYBR Color qPCR SuperMix |
EQ036-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix |
EQ036-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Color qPCR SuperMix |
EQ036 |
ELK Biotech |
5mL |
EUR 340 |
cDNA Synthesis SuperMix for qPCR |
20-abx09801920ulSystems |
Abbexa |
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- 100 rxns ×20 ul Systems
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HyperScript RT SuperMix for qPCR |
K1074-100 |
ApexBio |
100 rxn (20 uL/rxn) |
EUR 280 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR |
K1074-50 |
ApexBio |
50 rxn (20 uL/rxn) |
EUR 150 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
cDNA Synthesis SuperMix for qPCR |
abx098019-100l |
Abbexa |
100 µl |
EUR 825 |
cDNA Synthesis SuperMix for qPCR |
abx098019-1ml |
Abbexa |
1 ml |
EUR 2387.5 |
cDNA Synthesis SuperMix for qPCR |
abx098019-200l |
Abbexa |
200 µl |
EUR 1000 |
HiScript II Q RT SuperMix for qPCR |
R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 145 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
EQ035-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
EQ035-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
EQ035 |
ELK Biotech |
5mL |
EUR 340 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 861.6 |
|
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-100l |
Abbexa |
100 µl |
EUR 1025 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-1ml |
Abbexa |
1 ml |
Ask for price |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
abx098024-200l |
Abbexa |
200 µl |
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HiScript II Q Select RT SuperMix for qPCR |
R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 145 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
R423-01 |
Vazyme |
100rxns(20µl/rxn) |
EUR 326 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
R423-00-10rxns |
Vazyme |
10 rxns |
EUR 1.09 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
R423-01-100rxns |
Vazyme |
100 rxns |
EUR 337.36 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 217.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
abx098860-100l |
Abbexa |
100 µl |
EUR 1012.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
abx098860-1ml |
Abbexa |
1 ml |
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Uni Cell to cDNA Synthesis SuperMix for qPCR |
abx098860-200l |
Abbexa |
200 µl |
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HiScript III RT SuperMix for qPCR (+gDNA wiper) |
R323-00-10rxns |
Vazyme |
10 rxns |
EUR 1.09 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
R323-01-100rxns |
Vazyme |
100 rxns |
EUR 225.22 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
EQ033-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
EQ033-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
EQ033 |
ELK Biotech |
5mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
EQ034-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
EQ034-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
EQ034 |
ELK Biotech |
5mL |
EUR 340 |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
K1174-100 |
ApexBio |
100 rxns |
EUR 304 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HyperScript RT SuperMix for qPCR (with gDNA wiper) |
K1174-50 |
ApexBio |
50 rxns |
EUR 176 |
Description: High efficiency reverse transcription reaction premixed solution for two-step RT-qPCR method |
HiScript II Q RT SuperMix for qPCR (+gDNA wiper) |
R223-01 |
Vazyme |
100rxns(20µl/rxn) |
EUR 159.5 |
HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) |
R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 159.5 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
R333-01 |
Vazyme |
100 rxns (21 μl/rxn) |
EUR 217.5 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
R333-00-10rxns |
Vazyme |
10 rxns |
EUR 1.09 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
R333-01-100rxns |
Vazyme |
100 rxns |
EUR 225.22 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055200 |
Biochain |
200 reactions |
EUR 427 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055400 |
Biochain |
400 reactions |
EUR 682 |
Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055002 |
Biochain |
200 rxn |
EUR 426 |
Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
K5055004 |
Biochain |
400 rxn |
EUR 682 |
PCR SuperMix |
20-abx098887 |
Abbexa |
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2X PCR SuperMix |
MB200-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 11 |
2X PCR SuperMix |
MBS402025-100Reactions |
MyBiosource |
100Reactions |
EUR 175 |
2X PCR SuperMix |
MBS402025-4Reactions |
MyBiosource |
4Reactions |
EUR 160 |
Probe Direct SuperMix |
abx461057-1096tests |
Abbexa |
10 × 96 tests |
Ask for price |
Probe Direct SuperMix |
abx461057-596tests |
Abbexa |
5 × 96 tests |
EUR 2800 |
Probe Direct SuperMix |
abx461057-96tests |
Abbexa |
96 tests |
EUR 812.5 |
PCR SuperMix (-dye) |
20-abx098002 |
Abbexa |
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PCR SuperMix (+dye) |
20-abx098003 |
Abbexa |
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PCR SuperMix (-dye) |
abx098002-100l |
Abbexa |
100 µl |
EUR 237.5 |
PCR SuperMix (-dye) |
abx098002-200l |
Abbexa |
200 µl |
EUR 337.5 |
PCR SuperMix (+dye) |
abx098003-100l |
Abbexa |
100 µl |
EUR 237.5 |
PCR SuperMix (+dye) |
abx098003-200l |
Abbexa |
200 µl |
EUR 337.5 |
Plus PCR SuperMix |
20-abx098888 |
Abbexa |
-
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Evo? cDNA Supermix |
M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
each |
EUR 320.4 |
Plus PCR SuperMix |
abx098888-100l |
Abbexa |
100 µl |
EUR 300 |
Plus PCR SuperMix |
abx098888-200l |
Abbexa |
200 µl |
EUR 612.5 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
each |
EUR 346.8 |
cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
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- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
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cDNA Synthesis SuperMix |
abx098014-100l |
Abbexa |
100 µl |
EUR 725 |
cDNA Synthesis SuperMix |
abx098014-1ml |
Abbexa |
1 ml |
Ask for price |
cDNA Synthesis SuperMix |
abx098014-200l |
Abbexa |
200 µl |
EUR 812.5 |
T PCR SuperMix (-dye) |
20-abx098005 |
Abbexa |
-
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T PCR SuperMix (+dye) |
20-abx098006 |
Abbexa |
-
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T PCR SuperMix (-dye) |
abx098005-100l |
Abbexa |
100 µl |
EUR 262.5 |
T PCR SuperMix (-dye) |
abx098005-200l |
Abbexa |
200 µl |
EUR 462.5 |
T PCR SuperMix (+dye) |
abx098006-100l |
Abbexa |
100 µl |
EUR 262.5 |
T PCR SuperMix (+dye) |
abx098006-200l |
Abbexa |
200 µl |
EUR 462.5 |
AmpHS 2X PCR SuperMix |
MB203-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 11 |
EasyPfu PCR SuperMix (-dye) |
20-abx098009 |
Abbexa |
-
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FastPfu PCR SuperMix (-dye) |
20-abx098010 |
Abbexa |
-
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FastPfu PCR SuperMix (-dye) |
abx098010-100l |
Abbexa |
100 µl |
EUR 300 |
FastPfu PCR SuperMix (-dye) |
abx098010-200l |
Abbexa |
200 µl |
EUR 575 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054200 |
Biochain |
200 reactions |
EUR 468 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054400 |
Biochain |
400 reactions |
EUR 711 |
Library Amplification SuperMix |
20-abx098894 |
Abbexa |
-
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Library Amplification SuperMix |
abx098894-100l |
Abbexa |
100 µl |
EUR 737.5 |
Library Amplification SuperMix |
abx098894-200l |
Abbexa |
200 µl |
EUR 1137.5 |
OneScriptcDNA Synthesis SuperMix |
G451 |
ABM |
25 x 20 ul reactions |
EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
G452 |
ABM |
100 x 20 ul reactions |
EUR 169.2 |
PCR SuperMix for PAGE (+dye) |
20-abx098004 |
Abbexa |
-
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PCR SuperMix for PAGE (+dye) |
abx098004-100l |
Abbexa |
100 µl |
EUR 237.5 |
PCR SuperMix for PAGE (+dye) |
abx098004-200l |
Abbexa |
200 µl |
EUR 337.5 |
Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054002 |
Biochain |
200 rxn |
EUR 468 |
Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
K5054004 |
Biochain |
400 rxn |
EUR 711 |
Probe One-Step qRT-PCR SuperMix |
20-abx09804220ulSystems |
Abbexa |
-
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- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
|
Probe One-Step qRT-PCR SuperMix |
abx098042-100l |
Abbexa |
100 µl |
EUR 850 |
Probe One-Step qRT-PCR SuperMix |
abx098042-1ml |
Abbexa |
1 ml |
Ask for price |
Probe One-Step qRT-PCR SuperMix |
abx098042-200l |
Abbexa |
200 µl |
EUR 1400 |
OneScriptPlus cDNA Synthesis SuperMix |
G453 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
G454 |
ABM |
100 x 20 ul reactions |
EUR 202.8 |
OmniPCR Supermix w Fluorescent dye |
MBA01-0100 |
Bio-Helix |
100 rxns (2.5ml) |
EUR 16 |
EnTurbo™ SYBR Green PCR SuperMix |
EQ001-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix |
EQ001-5mL |
ELK Biotech |
5mL |
EUR 80 |
EnTurbo™ SYBR Green PCR SuperMix |
EQ001 |
ELK Biotech |
5mL |
EUR 340 |
Green Two-Step qRT-PCR SuperMix |
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 777.6 |
|
Green One-Step qRT-PCR SuperMix |
20-abx09803820ulSystems |
Abbexa |
-
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- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
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|
Green Two-Step qRT-PCR SuperMix |
abx098035-100l |
Abbexa |
100 µl |
EUR 925 |
Green Two-Step qRT-PCR SuperMix |
abx098035-1ml |
Abbexa |
1 ml |
Ask for price |
Green Two-Step qRT-PCR SuperMix |
abx098035-200l |
Abbexa |
200 µl |
Ask for price |
Green One-Step qRT-PCR SuperMix |
abx098038-100l |
Abbexa |
100 µl |
EUR 737.5 |
Green One-Step qRT-PCR SuperMix |
abx098038-1ml |
Abbexa |
1 ml |
Ask for price |
Green One-Step qRT-PCR SuperMix |
abx098038-200l |
Abbexa |
200 µl |
EUR 1050 |
AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X) |
MB204-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 13 |
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 727.2 |
|
Two-Step RT-PCR SuperMix (15 kb) |
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 910.8 |
|
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-100l |
Abbexa |
100 µl |
EUR 887.5 |
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-1ml |
Abbexa |
1 ml |
Ask for price |
Two-Step RT-PCR SuperMix (12 kb) |
abx098025-200l |
Abbexa |
200 µl |
Ask for price |
Two-Step RT-PCR SuperMix (15 kb) |
abx098026-100l |
Abbexa |
100 µl |
EUR 1087.5 |
Two-Step RT-PCR SuperMix (15 kb) |
abx098026-1ml |
Abbexa |
1 ml |
Ask for price |
Two-Step RT-PCR SuperMix (15 kb) |
abx098026-200l |
Abbexa |
200 µl |
Ask for price |
All-in-One cDNA Synthesis SuperMix |
B24403 |
Bimake |
200 ractions |
EUR 547.2 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
B24408 |
Bimake |
1000 ractions |
EUR 1743.6 |
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
OmniPCR HS Supermix w Fluorescent dye |
MBA03-0100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 16 |
The utmost restoration price of T. cruzi from inoculated açai juice was 82.50%. The restrict of detection and quantification in açai juice was 10 parasites/mL for RT-qPCR (mRNA-based) and qPCR (DNA-based). The RT-qPCR effectivity was estimated at 97.27% with an R2 of 0.994. The RT-qPCR was proven to have the ability to discriminate between viable and nonviable cells. This technique supplies a great tool for fast evaluation of low concentrations of viable T. cruzi in naturally contaminated meals samples, and may be utilized industrially as a high quality and safety technique.
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