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An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses
The real-time reverse transcription-polymerase chain reaction (real-time RT-qPCR) has become a leading technique for the detection and quantification of arboviruses, including chikungunya, dengue, and zika viruses. In this study, an updated real-time RT-qPCR assay was designed and evaluated together with a synthetic positive-control chimeric RNA for the simultaneous detection and Read more…